A Sacrifice-for-Survival Mechanism Protects Root Stem Cell Niche from Chilling Stress
A Sacrifice-for-Survival Mechanism Protects Root Stem Cell Niche from Chilling Stress
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DOI：
10.1016/j.cell.2017.06.002
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发表时间：
2017-06-29
期刊：
影响因子：
64.5
通讯作者：
Xu, Jian
中科院分区：
文献类型：
--
作者：
Hong, Jing Han;Savina, Maria;Xu, Jian
温度对植物和动物的发育有深远的影响，但它对干细胞行为和活动的影响仍然知之甚少。在这里，我们描述了拟南芥根对低温(低温但高于冰点)的反应。4摄氏度的低温胁迫主要导致根干细胞及其早期后代的DNA损伤。然而，只有新产生/分化的小柱干细胞子体(CSCD)优先以程序化方式死亡。抑制这些CSCD中的DNA损伤反应可以防止它们的死亡，但会使干细胞生态位更容易受到冷应激的影响。数学模型和实验验证表明，CSCD的死亡导致静止中心(QC)生长素最大值的重建，并在低温胁迫下维持功能性干细胞生态位的活性。这种机制提高了根部承受伴随的环境压力的能力，并在恢复最佳温度时恢复生长。
Temperature has a profound influence on plant and animal development, but its effects on stem cell behavior and activity remain poorly understood. Here, we characterize the responses of the Arabidopsis root to chilling (low but above-freezing) temperature. Chilling stress at 4 degrees C leads to DNA damage predominantly in root stem cells and their early descendants. However, only newly generated/differentiating columella stem cell daughters (CSCDs) preferentially die in a programmed manner. Inhibition of the DNA damage response in these CSCDs prevents their death but makes the stem cell niche more vulnerable to chilling stress. Mathematical modeling and experimental validation indicate that CSCD death results in the re-establishment of the auxin maximum in the quiescent center (QC) and the maintenance of functional stem cell niche activity under chilling stress. This mechanism improves the root's ability to withstand the accompanying environmental stresses and to resume growth when optimal temperatures are restored.