Cloning of a complete cDNA encoding human aromatase: immunochemical identification and sequence analysis.
Cloning of a complete cDNA encoding human aromatase: immunochemical identification and sequence analysis.
复制标题
编码人芳香酶的完整 cDNA 的克隆：免疫化学鉴定和序列分析。
DOI：
10.1016/s0006-291x(88)80903-3
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
N. Harada
中科院分区：
文献类型：
--
作者：
N. Harada
从人胎盘cDNA文库中克隆了一个编码人芳香化酶的完整cDNA克隆。制备针对由分离的克隆指定的多肽的抗体，并且人胎盘芳香化酶活性的Western印迹分析和抗体抑制实验证实了克隆为芳香化酶cDNA的鉴定。克隆的芳香化酶cDNA全长3030 bp，具有两个独特的EcoRI位点，其中3′端非编码区1397 bp，开放阅读框1509 bp，编码503个氨基酸残基，5′端非编码区124 bp。芳香化酶的氨基酸序列分析和芳香化酶与其他形式的细胞色素P-450的比较表明，这种酶是细胞色素P-450超家族的独特形式。
A complete cDNA clone encoding a human aromatase was isolated from a human placental cDNA library in λgtll. An antibody to the polypeptide specified by the isolated clone was prepared, and Western blot analysis and antibody inhibition experiments of human placental aromatase activity confirmed the identification of the clone as aromatase cDNA. The isolated aromatase cDNA clone of 3030 bp with two uniqueEcoRI sites contained a 3′-noncoding region of 1397 bp, an open reading frame of 1509 bp encoding 503 amino acid residues, and a 5′-noncoding region of 124 bp. Analysis of the amino acid sequence of aromatase and comparison of aromatase with other forms of cytochrome P-450 indicated that this enzyme is a unique form of the cytochrome P-450 superfamily.
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Evans,CT;Corbin,CJ;Saunders,CT;Merrill,JC;Simpson,ER;Mendelson,CR
通讯作者：
Mendelson,CR
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S