A decrease in the addition of new cells in the nucleus accumbens and prefrontal cortex between puberty and adulthood in male rats.
A decrease in the addition of new cells in the nucleus accumbens and prefrontal cortex between puberty and adulthood in male rats.
复制标题
雄性大鼠青春期和成年期之间伏核和前额皮质中新细胞的添加减少。
DOI：
10.1002/dneu.22160
复制
发表时间：
2014
影响因子：
3
通讯作者：
Sisk,CherylL
中科院分区：
文献类型：
--
作者：
Staffend,NancyA;Mohr,MargaretA;DonCarlos,LydiaL;Sisk,CherylL
青春期涉及社会行为，行为灵活性和适应性风险承担的转变，与大脑的结构重塑相一致。我们先前表明，新细胞被添加到与性行为相关的大脑区域，这表明细胞发生可能是青春期获得成人典型行为的机制。青春期细胞的增加是否发生在与行为灵活性或动机相关的大脑区域，以及这些模式在青春期和成年动物之间是否存在差异尚未确定。因此，我们评估了前额叶皮层和脑桥核中细胞增殖或存活的模式。对青春期和成年雄性大鼠注射溴脱氧尿苷（BrdU）。为了评估细胞增殖，在最后一次注射后24小时处死每组的一半动物。在最后一次注射后第30天处死剩余动物，以评价细胞存活。无论BrdU后存活时间如何，成年动物前额叶皮质中BrdU免疫反应（ir）细胞的密度均显著较低，而在脑桥核中，成年动物在较短的存活时间内BrdU免疫反应（ir）细胞的密度较低;然而，在较长的存活时间内，青春期和成年动物中BrdU免疫反应（ir）细胞的密度相等。这些数据提供的证据表明，青春期的细胞添加可能有助于重塑与行为灵活性和动机相关的大脑区域，这种细胞添加持续到成年期，尽管水平较低。年轻动物中较高水平的细胞增殖或存活可能反映了较高水平的可塑性，可能有助于青春期大脑的动态重塑。© 2013 Wiley Periodicals，Inc.开发神经生物学74：633-642，2014年
Adolescence involves shifts in social behaviors, behavioral flexibility, and adaptive risk‐taking that coincide with structural remodeling of the brain. We previously showed that new cells are added to brain regions associated with sexual behaviors, suggesting that cytogenesis may be a mechanism for acquiring adult‐typical behaviors during adolescence. Whether pubertal cell addition occurs in brain regions associated with behavioral flexibility or motivation and whether these patterns differ between pubertal and adult animals had not been determined. Therefore, we assessed patterns of cell proliferation or survival in the prefrontal cortex and nucleus accumbens. Pubertal and adult male rats were given injections of bromo‐deoxyuridine (BrdU). To assess cell proliferation, half of the animals from each group were sacrificed 24 h following the last injection. The remaining animals were sacrificed at Day 30 following the last injection to evaluate cell survival. Adult animals had significantly lower densities of BrdU‐immunoreactive (ir) cells in the prefrontal cortex, irrespective of post‐BrdU survival time, whereas in the nucleus accumbens, adult animals had a lower density of BrdU‐ir cells at the short survival time; however, the density of BrdU‐ir cells was equivalent in pubertal and adult animals at the longer survival time. These data provide evidence that cell addition during puberty may contribute to the remodeling of brain regions associated with behavioral flexibility and motivation, and this cell addition continues into adulthood, albeit at lower levels. Higher levels of cell proliferation or survival in younger animals may reflect a higher level of plasticity, possibly contributing to the dynamic remodeling of the pubertal brain. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 74: 633–642, 2014