Glutamate-induced intracellular calcium changes and neurotoxicity in cortical neuronsin vitro: Effect of chemical ischemia
Glutamate-induced intracellular calcium changes and neurotoxicity in cortical neuronsin vitro: Effect of chemical ischemia
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谷氨酸诱导的细胞内钙变化和体外皮质神经元的神经毒性：化学缺血的影响
DOI：
10.1016/0306-4522(94)90468-5
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发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
I.J Reynolds
中科院分区：
文献类型：
--
作者：
S. Rajdev;I.J Reynolds
为了研究钙在缺血期间神经元死亡中的作用，我们检查了在正常和缺血条件下暴露于谷氨酸（3 μM + 1 μM 甘氨酸）、NMDA（30 μM + 1 μM 甘氨酸）、红藻氨酸（100 μM）或高 K+（50 mM）5 分钟的单个大鼠前脑神经元的细胞内 Ca2+([Ca2+]i) 变化特征。测量的[Ca2+]i变化参数包括峰值[Ca2+]i水平、平台[Ca2+]i水平、[Ca2+]响应曲线下面积以及[Ca2+]i恢复到峰值响应的10%所需的时间。在正常条件下，所有研究的激动剂都产生相似的[Ca2+i变化。应用无葡萄糖缓冲液中的 5 mM KCN 模拟化学缺血，对 [Ca2+]i 的基础水平没有影响，但显着增强和延长所有激动剂产生的 [Ca2+]i 变化。然而，在毒性研究中，化学缺血仅显着增强谷氨酸和N-甲基-d-天冬氨酸的毒性。在相关研究中，所有死亡的神经元在丧失活力之前都表现出不可逆的继发[Ca2+iload]。这些研究表明，虽然Ca2+进入可能在神经元死亡中发挥关键作用，但初始[Ca2+]i变化的幅度并不能预测皮质神经元中激动剂的毒性。
To study the role of calcium in neuronal death during ischemia, we examined the characteristics of intracellular Ca2+([Ca2+]i) changes in single rat forebrain neurons exposed for 5 min to glutamate (3 μM + 1 μM glycine), NMDA (30 μM + 1 μM glycine), kainate (100 μM) or high K+(50 mM), under both normal and ischemie conditions. The parameters of [Ca2+]ichange measured included peak [Ca2+]ilevel, plateau [Ca2+]ilevel, area under the [Ca2+]iresponse curve and time taken by [Ca2+]ito recover to 10% of the peak response. Under normal conditions, all the agonists studied produced similar [Ca2+ichanges. Chemical ischemia simulated by application of 5 mM KCN in glucose-free buffer had no effect on the basal level of [Ca2+]i, but significantly enhanced and prolonged the [Ca2+ichanges produced by all the agonists. However, in toxicity studies, chemical ischemia significantly potentiated the toxicity of only glutamate andN-methyl-d-aspartate. In correlation studies, all the neurons which died displayed an irreversible secondary [Ca2+iload prior to loss of viability.These studies suggest that while Ca2+entry may play a critical role in neuronal death, the magnitude of initial [Ca2+]ichange does not predict the toxicity of an agonist in cortical neurons.
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影响因子：
--
作者：
J. Olney;Collins Rc;Sloviter Rs
通讯作者：
J. Olney;Collins Rc;Sloviter Rs
影响因子：
56.9
作者：
JONES, OT;KUNZE, DL;ANGELIDES, KJ
通讯作者：
ANGELIDES, KJ
影响因子：
56.9
作者：
WEISS, JH;HARTLEY, DM;CHOI, DW
通讯作者：
CHOI, DW
DOI：
--
发表时间：
1989-08
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Dean M. Hartley;D. Choi
通讯作者：
Dean M. Hartley;D. Choi