Superparamagnetic Iron Oxide Nanoparticles for Targeted Cell Seeding: Magnetic Patterning and Magnetic 3D Cell Culture
Superparamagnetic Iron Oxide Nanoparticles for Targeted Cell Seeding: Magnetic Patterning and Magnetic 3D Cell Culture
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DOI：
10.1002/adfm.202203672
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发表时间：
2022-08-03
影响因子：
19
通讯作者：
Janko, Christina
中科院分区：
文献类型：
--
作者：
Kappes, Mona;Friedrich, Bernhard;Janko, Christina
在再生医学中，细胞的非接触操作为组织工程提供了新的可能性。由于它们的物理化学性质，超顺磁性氧化铁纳米颗粒（SPION）用于生物医学中的各种应用，例如，作为药物转运体、造影剂或使细胞可被磁力吸引。SPION附着于细胞和/或被细胞吸收，使其能够用于过继免疫疗法或组织工程的磁性靶向。远程控制不同的“磁化”细胞类型可以用来构建多层组织，而不需要支架结构。在这里，SPION与各种涂层，如聚丙烯酸-共-马来酸（PAM），月桂酸（LA），月桂酸-人血清白蛋白（HSA）和柠檬酸盐磁化细胞的适用性与市售NanoShuttle-PL相比，设计用于磁性3D细胞培养。根据细胞标记的量，磁控制或多或少有效。特别是，PAM和柠檬酸盐包被的SPION实现了良好的细胞负载，并提供了细胞的磁性可控性。在2D细胞培养中，磁性货物允许细胞的模式化培养。在3D中，SPION能够并加速球体形成以及并行使用未加载和加载细胞的微图案化。
In regenerative medicine, noncontact manipulation of cells enables new possibilities for tissue engineering. Due to their physicochemical properties, superparamagnetic iron oxide nanoparticles (SPIONs) are used in biomedicine for various applications, e.g., as drug transporters, contrast agents or to make cells maneuverable by magnetic forces. SPIONs attached to and/or taken up by cells enable their magnetic targeting for adoptive immune therapies or tissue engineering. Remote control of different "magnetized" cell types can be used to construct multilayered tissues without the need for a scaffold structure. Here, the suitability of SPIONs with various coatings, such as polyacrylic acid-co-maleic acid (PAM), lauric acid (LA), lauric acid-human serum albumin (HSA), and citrate to magnetize cells is compared with the commercially available NanoShuttle-PL, designed for use in magnetic 3D cell cultures. Depending on the amount of cellular labeling, magnetic control is more or less effective. In particular, PAM- and citrate-coated SPIONs achieve good cellular loading and provide magnetic controllability of cells. In 2D cell culture, the magnetic cargo allows the patterned culture of cells. In 3D, SPIONs enable and accelerate spheroid formation as well as micropatterning using unloaded and loaded cells in parallel.