Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.
Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.
复制标题
DOI：
10.1084/jem.169.6.2233
复制
发表时间：
1989-06-01
期刊：
影响因子：
--
通讯作者：
Phillips JH
中科院分区：
文献类型：
--
作者：
Lanier LL;Testi R;Bindl J;Phillips JH
神经细胞粘附分子（N-CAM）是一种表达于神经和肌肉组织的膜糖蛋白，参与同型粘附相互作用。我们已经证明N-CAM也在造血细胞上表达，并且被抗Leu-19 mAb识别。Leu-19优先在介导MHC不受限制的细胞毒性的NK细胞和T淋巴细胞上表达，但也存在于一些髓性白血病细胞系上。在NK细胞、T细胞、KG1a.5造血细胞系和神经母细胞瘤细胞系上，Leu-19是具有N-连接的碳水化合物和丰富的唾液酸残基的约140-kD多肽。免疫共沉淀和肽图分析表明，白细胞和神经母细胞瘤细胞系上表达的Leu-19和N-CAM分子具有相似的结构。这些发现表明，白细胞上的Leu-19抗原可能参与细胞粘附，类似于神经细胞上N-CAM的功能。
Neural cell adhesion molecule (N-CAM) is a membrane glycoprotein expressed on neural and muscle tissues that is involved in homotypic adhesive interactions. We have demonstrated that N-CAM also is expressed on hematopoietic cells, and is recognized by the anti-Leu-19 mAb. Leu-19 is preferentially expressed on NK cells and T lymphocytes that mediate MHC-unrestricted cytotoxicity, but is also present on some myeloid leukemia cell lines. On NK cells, T cells, the KG1a.5 hematopoietic cell line, and a neuroblastoma cell line, Leu-19 is a approximately 140-kD polypeptide with N-linked carbohydrates and abundant sialic acid residues. Sequential immunoprecipitation and peptide mapping demonstrated that the Leu-19 and N-CAM molecules expressed on leukocyte and neuroblastoma cell lines are similar structures. These findings suggest that the Leu-19 antigen on leukocytes may be involved in cell adhesion, analogous to the function on N-CAM on neural cells.