N-cadherin promotes the commitment and differentiation of skeletal muscle precursor cells
N-cadherin promotes the commitment and differentiation of skeletal muscle precursor cells
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DOI：
10.1006/dbio.1997.8542
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发表时间：
1997-05-01
影响因子：
2.7
通讯作者：
Knudsen, KA
中科院分区：
文献类型：
--
作者：
GeorgeWeinstein, M;Gerhart, J;Knudsen, KA
具有形成骨骼肌潜能的细胞存在于原肠形成前的鸡胚中。肌肉分化开始于体节内原肠胚形成后。钙粘蛋白介导的粘附在骨骼肌前体细胞的承诺和分化的作用进行了研究，通过分析细胞间粘附分子的表达在培养的上胚层，节段板，体节细胞和通过确定的影响粘附干扰抗体的积累MyoD和肌节肌球蛋白。培养的原条期上胚层细胞下调E-cadherin和上调N-cadherin。这种钙粘蛋白表达的转换也发生在体内外胚层细胞进入原条时。虽然MyoD蛋白存在于具有N-或E-钙粘蛋白的细胞中，但仅具有N-钙粘蛋白的细胞分化为骨骼肌。与原条期的上胚层细胞不同，条前上胚层细胞在体外保持E-cadherin的表达。虽然大多数prestreak细胞含有MyoD，但只有少数合成肌球蛋白。用N-钙粘蛋白的功能干扰抗体处理原条阶段的上胚层细胞，导致肌球蛋白积累的抑制和MyoD细胞百分比的降低。节板和体节细胞与原条期上胚层细胞相似，在无血清培养基中培养时最能分化成骨骼肌。虽然N-钙粘蛋白的功能干扰抗体抑制肌球蛋白在这些中胚层细胞中的积累，MyoD阳性细胞的数量不受影响的体节培养物，只有部分减少节段板文化。这些结果表明，N-钙粘蛋白介导的细胞-细胞粘附参与肌肉前体的承诺和他们的终末分化。(C)北京：科学出版社.
Cells with the potential to form skeletal muscle are present in the chick embryo prior to gastrulation. Muscle differentiation begins after gastrulation within the somites. The role of cadherin-mediated adhesion in the commitment and differentiation of skeletal muscle precursor cells was examined by analyzing the expression of cell-cell adhesion molecules in cultures of epiblast, segmental plate, and somite cells and by determining the effects of adhesion-perturbing antibodies on the accumulation of MyoD and sarcomeric myosin. Cultured primitive streak stage epiblast cells downregulate E-cadherin and upregulate N-cadherin. This switch in cadherin expression also occurs in vivo as epiblast cells enter the primitive streak. Although MyoD protein is present in cells with N- or E-cadherin, only cells with N-cadherin differentiate into skeletal muscle. In contrast to the primitive streak stage epiblast cells, prestreak epiblast cells maintain the expression of E-cadherin in vitro. While the majority of prestreak cells contain MyoD, only a few synthesize myosin. Treatment of primitive streak stage epiblast cells with function-perturbing antibodies to N-cadherin resulted in an inhibition of myosin accumulation and a decrease in the percentage of cells with MyoD. Segmental plate and somite cells are similar to primitive streak stage epiblast cells in that most differentiated into skeletal muscle when cultured in serum-free medium. While function-perturbing antibodies to N-cadherin inhibited the accumulation of myosin in these mesoderm cells, the number of MyoD positive cells was unaffected in somite cultures and only partially reduced in segmental plate cultures. These results suggest that N-cadherin-mediated cell-cell adhesion is involved in both the commitment of muscle precursors and their terminal differentiation. (C) 1997 Academic Press.