Eukaryotic DNA Polymerases in Homologous Recombination.
Eukaryotic DNA Polymerases in Homologous Recombination.
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DOI：
10.1146/annurev-genet-120215-035243
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发表时间：
2016-11-23
影响因子：
11.1
通讯作者：
Heyer WD
中科院分区：
文献类型：
--
作者：
McVey M;Khodaverdian VY;Meyer D;Cerqueira PG;Heyer WD
同源重组（HR）是确保体细胞和减数分裂过程中基因组稳定性的核心过程。与同源重组相关的DNA合成在很大程度上决定了该过程的保真度。最近的一些研究表明，同源重组过程中的DNA合成比复制性DNA合成更具保守性、持续性更差且更易发生突变。在这篇综述中，我们描述了不同类型同源重组介导的DNA修复过程中DNA合成的机制特征，包括依赖合成的链退火、断裂诱导的复制以及减数分裂重组。我们强调了来自包括人类在内的多种真核生物的最新研究结果，这些结果表明复制性DNA聚合酶和跨损伤DNA聚合酶都参与了与同源重组相关的DNA合成。我们的重点是将关于同源重组过程中DNA聚合酶参与的新兴文献与这些修复机制的独特方面（包括突变发生和模板转换）相结合。
Homologous recombination (HR) is a central process to ensure genomic stability in somatic cells and during meiosis. HR-associated DNA synthesis determines in large part the fidelity of the process. A number of recent studies have demonstrated that DNA synthesis during HR is conservative, less processive, and more mutagenic than replicative DNA synthesis. In this review, we describe mechanistic features of DNA synthesis during different types of HR-mediated DNA repair, including synthesis-dependent strand annealing, break-induced replication, and meiotic recombination. We highlight recent findings from diverse eukaryotic organisms, including humans, that suggest both replicative and translesion DNA polymerases are involved in HR-associated DNA synthesis. Our focus is to integrate the emerging literature about DNA polymerase involvement during HR with the unique aspects of these repair mechanisms, including mutagenesis and template switching.