Dietary Supplementation with Sea Bass (Lateolabrax maculatus) Ameliorates Ulcerative Colitis and Inflammation in Macrophages through Inhibiting Toll-Like Receptor 4-Linked Pathways
Dietary Supplementation with Sea Bass (Lateolabrax maculatus) Ameliorates Ulcerative Colitis and Inflammation in Macrophages through Inhibiting Toll-Like Receptor 4-Linked Pathways
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DOI：
10.3390/ijms20122907
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发表时间：
2019-06-02
影响因子：
5.6
通讯作者：
Xu, Baojun
中科院分区：
文献类型：
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作者：
Chen, Jiali;Jayachandran, Muthukumaran;Xu, Baojun
花鲈（Lateolabrax maculatus）是一种常见的食用鱼。在传统的中医书籍中，已经表明鲈鱼可以用于管理许多炎症相关的病症。然而，对鲈鱼炎症的药理机制的研究仍然很少。因此，本研究旨在探讨鲈鱼抗炎活性的分子机制。采用葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎模型和脂多糖（LPS）激活的巨噬细胞模型评价鲈鱼的抗炎活性。在DSS喂养的小鼠中观察到低体重和短结肠长度，其在海鲈鱼处理后显著恢复。此外，结肠组织病理学评分显示，海鲈鱼处理的小鼠具有降低的隐窝损伤、局灶性炎症浸润和炎症程度，表明用海鲈鱼处理可以减轻肠道炎症。此外，体外研究还表明，鲈鱼可以通过抑制TLR 4相关通路来抑制LPS激活的巨噬细胞中的炎症介质。目前的研究结果表明，鲈鱼对TLR 4信号传导具有抑制作用;因此，它可能是治疗炎症相关疾病的有希望的候选者。有必要进一步论证海鲈鱼在治疗炎症相关疾病中的临床应用。
Sea bass (Lateolabrax maculatus) is a kind of food material commonly consumed in daily life. In traditional Chinese medicinal books, it has been indicated that sea bass can be applied for managing many inflammation-associated conditions. However, the studies on the pharmacological mechanisms of inflammation of sea bass remain scarce. Hence, this study aims to investigate the molecular mechanisms of the anti-inflammatory activity of sea bass. Anti-inflammatory activities of sea bass were assessed using dextran sulfate sodium (DSS)-induced colitis in a mice model and lipopolysaccharide (LPS)-activated macrophages model. Low body weight and short colon length were observed in DSS-fed mice that were significantly recovered upon sea bass treatments. Moreover, the colon histopathology score showed that sea bass-treated mice had decreased crypt damage, focal inflammation infiltration and the extent of inflammation, suggesting that treatment with sea bass could attenuate intestinal inflammation. In addition, the in-vitro study conjointly indicated that sea bass could suppress the inflammatory mediators in LPS-activated macrophage by inhibiting the TLR4-linked pathway. The present findings demonstrated that sea bass has an inhibitory effect on TLR4 signaling; thus, it could be a promising candidate for treating inflammation-associated conditions. A further justification for the clinical application of sea bass in treating inflammation-associated conditions is necessary.