Tissue engineering of an auricular cartilage model utilizing cultured chondrocyte-poly(L-lactide-ε-caprolactone) scaffolds
Tissue engineering of an auricular cartilage model utilizing cultured chondrocyte-poly(L-lactide-ε-caprolactone) scaffolds
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DOI：
10.1089/1076327041348527
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发表时间：
2004-05-01
影响因子：
--
通讯作者：
Landis, WJ
中科院分区：
文献类型：
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作者：
Isogai, N;Asamura, S;Landis, WJ
为了确定组织工程化耳软骨在体内的潜在发展，将来自牛前肢关节的关节软骨的软骨细胞接种在模塑成人耳形状的聚（L-乳酸-ε-己内酯）共聚物支架上。研究了相同形状的单独共聚物支架以进行比较。将接种软骨细胞的共聚物构建体和单独的支架分别植入无胸腺小鼠的背部皮瓣中长达40周。通过组织学和分子生物学技术对取回的标本进行了检查。植入10周后，甲苯胺蓝和番红-O红染色评估，细胞接种的结构开发软骨;血管，软骨膜样胶囊包围这些结构;和组织形成类似于最初塑造的耳廓形状。软骨基质形成增加，囊持续存在，并通过植入40周保持初始耳廓构型。软骨产生的存在与RT-PCR分析相关，RT-PCR分析显示在20周和40周时细胞接种标本中牛特异性II型胶原和聚集蛋白聚糖mRNA的表达。仅监测40周的共聚物支架未能形成软骨或限定的囊，并且不表达mRNA。广泛的血管化导致支架侵蚀，原始尺寸减小，轮廓和形状丧失。这些结果表明，在该植入模型中，接种有关节软骨细胞的聚（L-乳酸-ε-己内酯）共聚物在40周的时间内支持人耳形状的软骨的发育和维持。
To determine the potential development in vivo of tissue-engineered auricular cartilage, chondrocytes from articular cartilage of bovine forelimb joints were seeded on poly(L-lactic acid-epsilon-caprolactone) copolymer scaffolds molded into the shape of a human ear. Copolymer scaffolds alone in the same shape were studied for comparison. Chondrocyte-seeded copolymer constructs and scaffolds alone were each implanted in dorsal skin flaps of athymic mice for up to 40 weeks. Retrieved specimens were examined by histological and molecular techniques. After 10 weeks of implantation, cell-seeded constructs developed cartilage as assessed by toluidine blue and safranin-O red staining; a vascular, perichondrium-like capsule enveloped these constructs; and tissue formation resembled the auricular shape molded originally. Cartilage matrix formation increased, the capsule persisted, and initial auricular configuration was maintained through implantation for 40 weeks. The presence of cartilage production was correlated with RT-PCR analysis, which showed expression of bovine-specific type II collagen and aggrecan mRNA in cell-seeded specimens at 20 and 40 weeks. Copolymer scaffolds monitored only for 40 weeks failed to develop cartilage or a defined capsule and expressed no mRNA. Extensive vascularization led to scaffold erosion, decrease in original size, and loss of contour and shape. These results demonstrate that poly(L-lactic acid-epsilon-caprolactone) copolymer seeded with articular chondrocytes supports development and maintenance of cartilage in a human ear shape over periods to 40 weeks in this implantation model.