Glycyl-l-glutamine attenuates NPY-induced hyperphagia via the melanocortin system
Glycyl-l-glutamine attenuates NPY-induced hyperphagia via the melanocortin system
复制标题
甘氨酰-L-谷氨酰胺通过黑皮质素系统减弱 NPY 诱导的食欲亢进
DOI：
10.1016/j.neulet.2020.135303
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发表时间：
2020
影响因子：
2.5
通讯作者：
Bungo Takashi
中科院分区：
文献类型：
--
作者：
Shiraishi Jun-ichi;Yanagita Kouichi;Tanizawa Hiroshi;Bungo Takashi
本研究旨在确定甘氨酸-谷氨酰胺（Gly-Gln； β-内啡肽（30-31）），一种来源于β-内啡肽加工的非阿片肽，是否调节神经肽Y （NPY）诱导的雄性肉鸡饲养和下丘脑肽激素mRNA的表达。脑室内注射NPY （235 pmol）可在30 min内引起鸡的贪食反应。同时给药Gly-Gln （100 nmol）减弱了这种反应，诱导了30%的下降。这不能归因于甘氨酸-谷氨酰胺水解，因为甘氨酸（Gly）和谷氨酰胺（Gln）共同给药对npy诱导的贪食没有影响。单独注射Gly-Gln也没有效果。联合注射组下丘脑pro- opiomelanocortinmrna表达量显著高于NPY单独注射组。这些数据表明，内源性Gly-Gln可能通过中枢黑素皮质素系统调节雏鸡的摄食行为，并在能量代谢的神经活动中起反向调节作用。
This study aimed to determine whether glycyl-l-glutamine (Gly-Gln; β-endorphin (30–31)), a non-opioid peptide derived from β-endorphin processing, modulates neuropeptide Y (NPY)-induced feeding and hypothalamic mRNA expression of peptide hormones in male broiler chicks. Intracerebroventricular injection of NPY (235 pmol) generated a hyperphagic response inad libitumchicks within 30 min. Co-administration of Gly-Gln (100 nmol) attenuated this response, inducing a 30 % decrease. This was not attributable to Gly-Gln hydrolysis because co-administration of glycine (Gly) and glutamine (Gln) had no effect on NPY-induced hyperphagia. Gly-Gln injected alone also showed no effect. The hypothalamicpro-opiomelanocortinmRNA expression in the co-injection group was significantly higher than that in the NPY alone group. These data indicate that endogenous Gly-Gln may contribute to regulate feeding behaviorviathe central melanocortin system in chicks and acts as a counter regulator of the neural activity in energy metabolism.