Rapid resolution of carbohydrate isomers by electrospray ionization ambient pressure ion mobility spectrometry-time-of-flight mass spectrometry (ESI-APIMS-TOFMS)
Rapid resolution of carbohydrate isomers by electrospray ionization ambient pressure ion mobility spectrometry-time-of-flight mass spectrometry (ESI-APIMS-TOFMS)
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DOI：
10.1016/j.jasms.2007.04.007
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发表时间：
2007-07-01
影响因子：
3.2
通讯作者：
Hill, Herbert H., Jr.
中科院分区：
文献类型：
--
作者：
Dwivedi, Prabha;Bendiak, Brad;Hill, Herbert H., Jr.
碳水化合物是一组极其复杂的异构体分子，难以通过质谱法在气相中进行分析，因为（1）MSn连续阶段的前体离子和产物离子通常是异构体的混合物，以及（2）关于较大分子内单糖的异头构型和特定立体化学变体的位置的详细信息不可能以一般方式获得。在此，它表明，气相分析通过直接组合的电喷雾电离，环境压力离子迁移谱，和飞行时间质谱（ESI-APIMS-TOFMS）提供足够的分辨率，以分离不同的异头甲基糖苷和分离不同的立体异构的甲基糖苷具有相同的异头构型。还原糖通常分解成多于一个峰，这可能代表具有不同端基异构体构型和/或环形式的环状物质的分离。分离的程度，无论是与甲基糖苷和还原糖，显着影响的漂移气体的性质和加合金属离子或离子络合物的性质。研究表明，ESI-APIMS-TOFMS是一种快速、有效的甲基糖苷异构体和单糖的分离分析技术，可用于区分具有不同端基异构构型的糖苷。
Carbohydrates are an extremely complex group of isomeric molecules that have been difficult to analyze in the gas phase by mass spectrometry because (1) precursor ions and product ions to successive stages of MSn are frequently mixtures of isomers, and (2) detailed information about the anomeric configuration and location of specific stereochemical variants of monosaccharides within larger molecules has not been possible to obtain in a general way. Herein, it is demonstrated that gas-phase analyses by direct combination of electrospray ionization, ambient pressure ion mobility spectrometry, and time-of-flight mass spectrometry (ESI-APIMS-TOFMS) provides sufficient resolution to separate different anomeric methyl glycosides and to separate different stereoisomeric methyl glycosides having the same anomeric configuration. Reducing sugars were typically resolved into more than one peak, which might represent separation of cyclic species having different anomeric configurations and/or ring forms. The extent of separation, both with methyl glycosides and reducing sugars, was significantly affected by the nature of the drift gas and by the nature of an adducting metal ion or ion complex. The study demonstrated that ESI-APIMS-TOFMS is a rapid and effective analytical technique for the separation of isomeric methyl glycosides and simple sugars, and can be used to differentiate glycosides having different anomeric configurations.