Localization of α1-2 Fucose Glycan in the Mouse Olfactory Pathway
Localization of α1-2 Fucose Glycan in the Mouse Olfactory Pathway
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DOI：
10.1159/000447009
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发表时间：
2017-01-01
影响因子：
2.7
通讯作者：
Kitamura, Nobuo
中科院分区：
文献类型：
--
作者：
Kondoh, Daisuke;Kamikawa, Akihiro;Kitamura, Nobuo
嗅觉系统中的糖缀合物在神经元形成中起关键作用，并且α 1-2岩藻糖（α 1-2Fuc）聚糖介导神经突生长和突触可塑性。使用荆豆凝集素-I（UEA-I）检测的小鼠嗅觉系统中a1-2Fuc聚糖的组织化学结果各不相同。本研究使用UEA-I对3-4月龄雄性和雌性ICR和C57 BL/6 J小鼠的主要嗅觉和犁鼻通路进行了组织化学评估。荆豆凝集素-I与大多数受体细胞反应，主要分布在嗅上皮基底部。主嗅球的嗅神经层和嗅球层呈点状UEA-Ⅰ阳性染色，阳性纤维从嗅球层向内丛状层散在分布。外侧嗅束和吻侧迁移流也呈UEA-I阳性。我们确定了浅层shortaxon细胞，外丛状层的中间神经元，外部，中间和内部簇状细胞，僧帽细胞和颗粒细胞作为UEA-I阳性纤维在主嗅球的起源。嗅前核、梨状前皮质和嗅结节UEA-Ⅰ均为阴性。犁鼻上皮和副嗅球的肾小球中大多数受体细胞呈UEA-I阳性。我们的研究结果表明，α 1-2Fuc聚糖位于主要嗅觉系统的初级和次级途径，但不是三级途径，主要嗅球的局部回路和犁鼻系统的初级途径，但不是次级途径。(C)2016 S. Karger AG，巴塞尔
Glycoconjugates in the olfactory system play critical roles in neuronal formation, and alpha 1-2 fucose (alpha 1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of a1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This study histochemically assessed the main olfactory and vomeronasal pathways in male and female ICR and C57BL/6J mice aged 3-4 months using UEA-I. Ulex europaeus agglutinin-I reacted with most receptor cells arranged mainly at the basal region of the olfactory epithelium. The olfactory nerve layer and glomerular layer of the main olfactory bulb were speckled with positive UEA-I staining, and positive fibers were scattered from the glomerular to the internal plexiform layer. The lateral olfactory tract and rostral migratory stream were also positive for UEA-I. We identified superficial shortaxon cells, interneurons of the external plexiform layer, external, middle and internal tufted cells, mitral cells and granule cells as the origins of the UEA-I-positive fibers in the main olfactory bulb. The anterior olfactory nucleus, anterior piriform cortex and olfactory tubercle were negative for UEA-I. Most receptor cells in the vomeronasal epithelium and most glomeruli of the accessory olfactory bulb were positive for UEA-I. Our findings indicated that alpha 1-2Fuc glycan is located within the primary and secondary, but not the ternary, pathways of the main olfactory system, in local circuits of the main olfactory bulb and within the primary, but not secondary, pathway of the vomeronasal system. (C) 2016 S. Karger AG, Basel