Evaluation of Pyrosequencing technology for the identification of clinically relevant non-dematiaceous yeasts and related species.
Evaluation of Pyrosequencing technology for the identification of clinically relevant non-dematiaceous yeasts and related species.
复制标题
焦磷酸测序技术用于鉴定临床相关非暗色酵母及相关物种的评估。
DOI：
10.1007/s10096-008-0510-x
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Murray,PR
中科院分区：
文献类型：
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作者：
Montero,CI;Shea,YR;Jones,PA;Harrington,SM;Tooke,NE;Witebsky,FG;Murray,PR
Pyrosequencing was used to identify 133 isolates of clinically relevant non-dematiaceous yeasts. These included 97 ATCC strains (42 type strains), seven UAMH strains, and 29 clinical isolates. Isolates belonged to the following genera:Candida(18 species),Trichosporon(10),Cryptococcus(7),Malassezia(3),Rhodotorula(2),Geotrichum(1),Blastoschizomyces(1), andKodamaea(1). Amplicons of a hyper-variable ITS region were obtained and analyzed using Pyrosequencing technology. The data were evaluated by a BLAST search against the GenBank database and correlated with data obtained by conventional cycle sequencing of the ITS1–5.8S–ITS2 region. Cycle sequencing identified 78.9% of the isolates to the species level. Pyrosequencing technology identified 69.1%. In 90.1% of all of the strains tested, the identification results of both sequencing methods were identical. MostCandidaisolates can be identified to the species level by Pyrosequencing.Trichosporonspecies and someCryptococcusspecies cannot be differentiated at the species level. Pyrosequencing can be used for the reliable identification of most commonly isolated non-dematiaceous yeasts, with a reduction of cost per identification compared to conventional sequencing.
