Particulate matter from Saudi Arabia induces genes involved in inflammation, metabolic syndrome and atherosclerosis.
Particulate matter from Saudi Arabia induces genes involved in inflammation, metabolic syndrome and atherosclerosis.
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DOI：
10.1080/15287394.2014.892446
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Costa M
中科院分区：
文献类型：
--
作者：
Brocato J;Sun H;Shamy M;Kluz T;Alghamdi MA;Khoder MI;Chen LC;Costa M
空气中的颗粒物（PM）暴露是一个主要的环境健康问题，并与代谢紊乱有关，如心血管疾病（CVD）和糖尿病，这些疾病在沙特阿拉伯王国呈上升趋势。本研究调查了从沙特阿拉伯吉达收集的PM10给药产生的小鼠肺基因表达的变化。通过抽吸将FVB/N小鼠暴露于100 µg PM10或水，并在24小时后实施安乐死。收集支气管肺泡灌洗液（BALF）并分析中性粒细胞浓度和TNF-α和IL-6水平。从肺中提取RNA，并使用Affyssin Mouse Gene 1.0 ST Array分析全转录物。暴露于PM10的小鼠表现出中性粒细胞浓度增加和TNF-α和IL-6水平升高。基因表达分析显示，暴露于PM10的小鼠显示202个基因显著上调，40个基因显著下调。PM10诱导的基因与炎症、胆固醇和脂质代谢以及动脉粥样硬化有关。这是第一项证明沙特阿拉伯PM10增加体内基因表达的研究，这些基因位于与代谢综合征和动脉粥样硬化相关的疾病途径中。
Airborne particulate matter (PM) exposure is a major environmental health concern and is linked to metabolic disorders, such as cardiovascular diseases (CVD) and diabetes, which are on the rise in the Kingdom of Saudi Arabia. This study investigated changes in mouse lung gene expression produced by administration of PM10 collected from Jeddah, Saudi Arabia. FVB/N mice were exposed to 100 µg PM10 or water by aspiration and euthanized 24 hr later. The bronchoalveolar lavage fluid (BALF) was collected and analyzed for neutrophil concentration and TNF-α and IL-6 levels. RNA was extracted from the lungs and whole transcript was analyzed using Affymetrix Mouse Gene 1.0 ST Array. Mice exposed to PM10 displayed an increase in neutrophil concentration and elevated TNF-α and IL-6 levels. Gene expression analysis revealed that mice exposed to PM10 displayed 202 genes that were significantly up-regulated and 40 genes that were significantly down-regulated. PM10 induced genes involved in inflammation, cholesterol and lipid metabolism, as well as atherosclerosis. This is the first study to demonstrate that Saudi Arabia PM10 increases in vivo expression of genes located in pathways associated with diseases involving metabolic syndrome and atherosclerosis.
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影响因子：
4
作者：
Fasshauer, M;Klein, J;Paschke, R
通讯作者：
Paschke, R
DOI：
10.4049/jimmunol.1100500
发表时间：
2011-07-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ather JL;Ckless K;Martin R;Foley KL;Suratt BT;Boyson JE;Fitzgerald KA;Flavell RA;Eisenbarth SC;Poynter ME
通讯作者：
Poynter ME
影响因子：
5
作者：
Bombini, G;Canetti, C;Cunha, FQ
通讯作者：
Cunha, FQ
影响因子：
10
作者：
Araujo JA;Nel AE
通讯作者：
Nel AE
DOI：
10.1080/15287394.2013.801763
发表时间：
2013-05-01
影响因子：
2.6
作者：
Chiu, Hui-Fen;Tsai, Shang-Shyue;Yang, Chun-Yuh
通讯作者：
Yang, Chun-Yuh