Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source.
Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source.
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DOI：
10.1038/sdata.2016.64
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发表时间：
2016-08-01
期刊：
影响因子：
9.8
通讯作者：
Zook J
中科院分区：
文献类型：
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作者：
Munke A;Andreasson J;Aquila A;Awel S;Ayyer K;Barty A;Bean RJ;Berntsen P;Bielecki J;Boutet S;Bucher M;Chapman HN;Daurer BJ;DeMirci H;Elser V;Fromme P;Hajdu J;Hantke MF;Higashiura A;Hogue BG;Hosseinizadeh A;Kim Y;Kirian RA;Reddy HK;Lan TY;Larsson DS;Liu H;Loh ND;Maia FR;Mancuso AP;Mühlig K;Nakagawa A;Nam D;Nelson G;Nettelblad C;Okamoto K;Ourmazd A;Rose M;van der Schot G;Schwander P;Seibert MM;Sellberg JA;Sierra RG;Song C;Svenda M;Timneanu N;Vartanyants IA;Westphal D;Wiedorn MO;Williams GJ;Xavier PL;Yoon CH;Zook J
在直线加速器相干光源（LCLS）上使用相干X射线成像（CXI）仪器记录来自水稻矮缩病毒（RDV）的单粒子衍射成像数据。选择RDV是因为它是一个特征良好的模型系统，可用于原理验证实验，系统优化和算法开发。RDV是一种直径约为70 nm的二十面体病毒，在LCLS的CXI仪器上雾化并注入直径约为0.1 μm的聚焦硬X射线束中。记录来自RDV的衍射图案，其信号为5.9毫微克。衍射数据可通过相干X射线成像数据库（CXIDB）作为算法开发的资源，其内容在这里描述。
Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.