TRANSFER RNA-LIKE STRUCTURES OF PLANT VIRAL RNAS - CONFORMATIONAL REQUIREMENTS FOR ADENYLATION AND AMINOACYLATION
TRANSFER RNA-LIKE STRUCTURES OF PLANT VIRAL RNAS - CONFORMATIONAL REQUIREMENTS FOR ADENYLATION AND AMINOACYLATION
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DOI：
10.1002/j.1460-2075.1983.tb01556.x
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发表时间：
1983-01-01
期刊：
影响因子：
11.4
通讯作者：
HAENNI, AL
中科院分区：
文献类型：
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作者：
JOSHI, RL;JOSHI, S;HAENNI, AL
溴病毒和黄瓜病毒RNA含有tRNA样结构作为其基因组的组成部分。该结构位于病毒RNA的3“末端，是酪氨酸的受体。代表性的病毒RNA的3“区域已经被测序，并且对于这些3”末端提出了非常非正统的二级折叠。问题仍然是这些结构如何被tRNA特异性酶识别。确定了形成酪氨酰-tRNA合成酶和/或tRNA核苷酸转移酶识别的结构所需的来自雀麦花叶病毒和蚕豆斑驳病毒RNA的3“末端的最小核苷酸数。所获得的结果描绘了tRNA样区域的长度，并表明tRNA样结构的5“区域参与了氨基酸茎的形成。这导致了对这些tRNA样区域的“L”形二级结构的建议。
Bromo- and cucumovirus RNA contain a tRNA-like structure as an integral part of their genome. This structure is located at the 3'' end of the viral RNA and is an acceptor of tyrosine. The 3'' regions of representative viral RNA have been sequenced and quite unorthodox secondary foldings have been proposed for these 3'' ends. The question remained as to how these structures could be recognized by tRNA-specific enzymes. The minimum number of nucleotides from the 3'' end of the brome mosaic virus and broad bean mottle virus RNA required for the formation of structures recognized by the tyrosyl-tRNA synthetase and/or the tRNA nucleotidyltransferase was established. The results obtained delineate the length of the tRNA-like region and indicate that the 5'' region of the tRNA-like structure participates in the formation of the amino acid stem. This led to a proposal for an ''L''-shaped secondary structure for these tRNA-like regions.