A gene encoding a tyrosine tRNA synthetase is located near sacS in Bacillus subtilis.
A gene encoding a tyrosine tRNA synthetase is located near sacS in Bacillus subtilis.
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DOI：
10.3109/10425179109020780
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
Dedonder, R
中科院分区：
文献类型：
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作者：
Glaser, P;Kunst, F;Dedonder, R
在尝试测序整个枯草芽孢杆菌基因组的框架内，将B. sacS基因座附近的枯草杆菌染色体已被测序。它包含五个完整的编码序列，包括sacY序列，三个未知的CDS和一个编码酪氨酸tRNA合成酶的序列。相应的CDS编码功能性合成酶已经通过具有热敏性tRNA合成酶的大肠杆菌突变体的互补来证明。在B中插入卡那霉素抗性盒。然而，在相应的基因座上的枯草杆菌染色体上没有产生明显的表型，表明该合成酶是不稳定的。最后讨论了已知的酪氨酸和色氨酸tRNA合成酶之间的系统发育关系。
Within the frame of an attempt to sequence the whole Bacillus subtilis genome, a region of 5.5 kbp of the B. subtilis chromosome near the sacS locus has been sequenced. It contains five complete coding sequences, including the sequence of sacY, three unknown CDS and a sequence coding for a tyrosine tRNA synthetase. That the corresponding CDS encodes a functional synthetase has been demonstrated by complementation of an Escherichia coli mutant possessing a thermosensitive tRNA synthetase. Insertion of a kanamycin resistance cassette in the B. subtilis chromosome at the corresponding locus resulted, however, in no apparent phenotype, demonstrating that this synthetase is dispensable. Finally phylogenetic relationships between known tyrosine and tryptophan tRNA synthetases are discussed.