The occurrence and transmission characteristics of airborne H9N2 avian influenza virus
The occurrence and transmission characteristics of airborne H9N2 avian influenza virus
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DOI：
10.2376/0005-9366-124-136
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发表时间：
2011-03-01
影响因子：
0.4
通讯作者：
Zhang, Hongshuang
中科院分区：
文献类型：
--
作者：
Yao, Meiling;Zhang, Xingxiao;Zhang, Hongshuang
为了更好地了解H9N2禽流感病毒（AIV）的传播途径，我们进行了两次重复试验，观察了特定无病原体鸡的气溶胶感染和直接接触过程。15只鸡（G1）接种H9N2 AIV，与另外15只鸡（G2）一起饲养在同一个正负压隔离器(A)中。15只鸡（G3）饲养在另一个隔离器(B)中，该隔离器与A相连，使空气从A向B单向流动。采集空气、口咽拭子和直肠拭子，并采集血样，检测雾化病毒、病毒脱落和血清转化。在接种后2-3天开始检测到AIV气溶胶，在接种后7天达到峰值。G2组所有鸡均有病毒脱落，而G3组只有部分病毒脱落（Ti: 87%,T2: 80%）。抗体最初在4-5 dpi处检测到，在14-21 dpi处达到峰值。结果表明，H9N2 AIV可通过气溶胶接触和直接接触两种途径传播。
To better understand the transmission route of H9N2 avian influenza virus (AIV), two duplicate trials were conducted to observe the process of aerosol infection and direct contact in specific pathogen free chickens. Fifteen chickens (G1) were inoculated with H9N2 AIV and housed together with another 15 chickens (G2) in the same positive-negative-pressure isolator (A). Fifteen chickens (G3) were bred in another isolator (B) which was connected with A so that air could flow unidi-rectionally from A to B. Air, oropharyngeal and cloacal swabs, and blood samples were collected for the detection of aerosolized virus, virus shedding, and seroconversion. AIV aerosols were initially detected at day 2-3 post inoculation (dpi), reaching peak concentrations at 7 dpi. Virus shedding was detected in all chickens of G2, but only in a part in G3 (Ti: 87%,T2: 80%). Antibodies were initially detected at 4-5 dpi, peaking at 14-21 dpi. The results showed that H9N2 AIV could be transmitted by both aerosol exposure and direct contact.