Myogenic regulatory factors and muscle differentiation during ontogeny in fish
Myogenic regulatory factors and muscle differentiation during ontogeny in fish
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DOI：
10.1111/j.1095-8649.1999.tb01042.x
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发表时间：
1999-12
影响因子：
2
通讯作者：
S. Watabe
中科院分区：
文献类型：
--
作者：
S. Watabe
利用逆转录聚合酶链反应（RT-PCR）技术从鲤鱼幼鱼和胚胎中克隆了肌细胞生成调节因子myogenin、MyoD和myf-5的cDNA克隆，并从鲤鱼成鱼cDNA文库中鉴定了MEF 2 cDNA。在鲤鱼体节形成过程中，myf-5是最先表达的因子，其次是MEF 2C和MyoD，然后是myogenin和MEF 2A，最后是骨骼肌肌球蛋白重链和α-actin。本研究还通过北方印迹分析，研究了水温20 ~ 30°C驯化过程中鲤鱼快肌肌肌球蛋白重链（MyHC）亚型mRNA水平的变化及其与MyoD家族和MEF 2家族成员mRNA水平的关系。有一个显着减少的转录的MyHC亚型主要表达在冷驯化鲤鱼和一个显着增加的转录的MyHC亚型主要表达在热驯化鲤鱼。在相同的驯化期内，myogenin，MEF 2A和MEF 2C的转录水平发生了显着变化，而MyoD的转录本是相当恒定的。本文还讨论了MRFs及其相关转录因子在鱼类肌肉分化中的可能作用。
cDNA clones encoding the myogenic regulatory factors, myogenin, MyoD and myf-5 were cloned by reverse-transcription polymerase chain reaction from larvae and embryos of the common carp MEF2 cDNAs were also identified from an adult carp cDNA library. During the period of somite formation for carp, myf-5 was the first factor to be expressed followed by MEF2C and MyoD, then myogenin and MEF2A, and finally skeletal myosin heavy chain and α-actin. This study also examined, by Northern blot analysis, the accumulated mRNA levels of myosin heavy chain (MyHC) isoforms in carp fast skeletal muscle during water temperature acclimation from 20 to 30°C in relation to those of MyoD family and MEF2 family members. There was a dramatic decrease in the transcripts of the MyHC isoform predominantly expressed in cold-acclimated carp and a significant increase in the transcripts of the MyHC isoform predominantly expressed in warm-acclimated carp. Over the same acclimation period, the transcription levels of myogenin, MEF2A and MEF2C changed significantly, whereas MyoD transcripts were rather constant. The possible roles of MRFs and related transcription factors in muscle differentiation of fish are discussed.