Plasmid vectors for the analysis of protein-induced DNA bending.
Plasmid vectors for the analysis of protein-induced DNA bending.
复制标题
用于分析蛋白质诱导的 DNA 弯曲的质粒载体。
DOI：
10.1007/978-1-60327-015-1_32
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Adhya,Sankar
中科院分区：
文献类型：
--
作者：
Zwieb,Christian;Adhya,Sankar
Bending is not only required to accommodate DNA within the cell but also is a mechanism used by proteins to initiate DNA replication, transcription, and recombination. Determining the angles by which regulatory DNA segments deviate from linearity upon binding of proteins is a necessary step toward a better understanding of a large number of essential biological functions. The pBend plasmids contain duplicate sets of restriction sites and, when combined with “gel shift” experiments, allow the straightforward determination of the bending angle in a DNA molecule. The steps for successfully carrying out a binding/bending experiment are described. They include the cloning of the protein-binding site into the chosen pBend vector, the isolation of a series of DNA fragments with identical in length but variable placing of the protein-binding site, and the gel electrophoretic analysis of the free and protein-bound fragments.
