Comparison of expression patterns between CREB family transcription factor OASIS and proteoglycan core protein genes during murine tooth development
Comparison of expression patterns between CREB family transcription factor OASIS and proteoglycan core protein genes during murine tooth development
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DOI：
10.1007/s00429-003-0311-z
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发表时间：
2003-04-01
期刊：
影响因子：
--
通讯作者：
Wanaka, A
中科院分区：
文献类型：
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作者：
Hikake, T;Mori, T;Wanaka, A
编码 CREB/ATF 家族成员的转录因子 OASIS 基因在小鼠胚胎的唾液腺、软骨和牙胚中特异性表达。本研究通过原位杂交组织化学方法比较了小鼠下颌第一磨牙牙胚中OASIS mRNA与主要脊椎动物蛋白多糖的表达模式，蛋白多糖可能是OASIS的下游基因。在帽状期和钟状期 (E14.5-E18.5) 的内釉质上皮中观察到 OASIS mRNA 表达，在分化期的前成牙本质细胞 (E18.5-P0) 和早期分泌期的分化成牙本质细胞 (P2.5-P4.5) 中观察到 OASIS mRNA 表达。蛋白多糖（多功能蛋白聚糖、核心蛋白聚糖、双糖链蛋白聚糖、磷脂酰肌醇聚糖、多糖蛋白聚糖-1 和多糖蛋白聚糖-3）在牙胚中以各种模式表达。核心蛋白聚糖、双聚糖、syndecan-1 和 syndecan-3 显示与 OASIS 重叠的基因表达。尤其是decorin和syndecan-3的表达模式在时间和空间上与OASIS的表达模式完全一致。这些结果表明OASIS基因可能与蛋白多糖表达有关，并且可能在成牙本质细胞和内釉质上皮细胞的分化中发挥重要作用。
The transcription factor OASIS gene, which encodes for a CREB/ATF family member, is specifically expressed in the salivary gland, the cartilage and the tooth germs of the mouse embryo. In the present study, the expression patterns were compared between OASIS mRNA and major vertebrate proteoglycans, which might be the downstream genes of OASIS in the tooth germs of mouse first mandibular molars, through in situ hybridization histochemistry. OASIS mRNA expression was observed in the inner enamel epithelium during the cap and bell stages (E14.5-E18.5) in the preodontoblasts during differentiation stage (E18.5-P0) and in the differentiating odontoblasts during the early secretory stage (P2.5-P4.5). Proteoglycans (versican, decorin, biglycan, glypican, syndecan-1, and syndecan-3) were expressed in the tooth germs in various patterns. Decorin, biglycan, syndecan-1 and syndecan-3 showed gene expressions overlapping with OASIS. Especially the expression pattern of decorin and syndecan-3 coincided temporally and spatially exactly with that of OASIS. These results suggest that the OASIS gene might be related to proteoglycan expression and may play an important role in the differentiation of the odontoblast and cells in inner enamel epithelium.