A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink
A novel, disposable, screen-printed amperometric biosensor for glucose in serum fabricated using a water-based carbon ink
复制标题
DOI：
10.1016/j.bios.2005.01.003
复制
发表时间：
2005-11-15
影响因子：
12.6
通讯作者：
Hart, JP
中科院分区：
文献类型：
--
作者：
Crouch, E;Cowell, DC;Hart, JP
利用水基碳墨制作了丝网印刷电流型葡萄糖生物传感器。酶葡萄糖氧化酶（GOD）和电催化剂酞菁钴与碳墨混合之前的丝网印刷过程，因此，生物传感器的制备在一个步骤的制造过程。通过研究pH值、缓冲液强度和外加电位对分析响应的影响，实现了生物传感器性能的优化。在最佳条件下进行校准研究，使用搅拌溶液中的电流分析法，工作电位为+500 mV vs. SCE。灵敏度为1170 nA mM（-1），线性范围为0.025-2 mM;前者代表检测限。通过对牛血清样品进行重复测定来评价一次性安培型生物传感器。这是通过多个标准加入的方法实现的，包括对可氧化血清组分产生的背景电流的校正。计算的平均血清浓度为8.63 mM，与供应商的值8.3 mM比较良好;计算的变异系数为3.3%（n = 6）。(c)2005 Elsevier B. V.保留所有权利。
Screen-printed amperometric glucose biosensors have been fabricated using a water-based carbon ink. The enzyme glucose oxidase (GOD) and the electrocatalyst cobalt phthalocyanine were mixed with the carbon ink prior to the screen-printing process; therefore, biosensors are prepared in a one-step fabrication procedure. Optimisation of the biosensor performance was achieved by studying the effects of pH, buffer strength, and applied potential on the analytical response. Calibration studies were performed under optimum conditions, using amperometry in stirred solution, with an operating potential of +500 mV versus SCE. The sensitivity was found to be 1170 nA mM(-1), with a linear range of 0.025-2 mM; the former represents the detection limit. The disposable amperometric biosensor was evaluated by carrying out replicate determinations on a sample of bovine serum. This was achieved by the method of multiple standard additions and included a correction for background currents arising from oxidisable serum components. The mean serum concentration was calculated to be 8.63 mM and compared well with the supplier's value of 8.3 mM; the coefficient of variation was calculated to be 3.3% (n = 6). (c) 2005 Elsevier B.V. All rights reserved.