INFLUENCE OF HOST FACTORS ON NEUROINVASIVENESS OF VESICULAR STOMATITIS VIRUS : II. EFFECT OF AGE ON THE INVASION OF THE PERIPHERAL AND CENTRAL NERVOUS SYSTEMS BY VIRUS INJECTED INTO THE LEG MUSCLES OR THE EYE.
INFLUENCE OF HOST FACTORS ON NEUROINVASIVENESS OF VESICULAR STOMATITIS VIRUS : II. EFFECT OF AGE ON THE INVASION OF THE PERIPHERAL AND CENTRAL NERVOUS SYSTEMS BY VIRUS INJECTED INTO THE LEG MUSCLES OR THE EYE.
复制标题
DOI：
10.1084/jem.66.1.35
复制
发表时间：
1937-06-30
期刊：
影响因子：
--
通讯作者：
Olitsky PK
中科院分区：
文献类型：
--
作者：
Sabin AB;Olitsky PK
1.将水泡性口炎病毒注射到年轻小鼠的腿部肌肉中，会引起接种肢体的弛缓性麻痹，这是一种疾病的第一个临床症状，这种疾病总是致命的;同样，注射最大剂量病毒的老年小鼠也能存活下来，没有表现出任何疾病的迹象。2.在幼龄小鼠中，病毒在接种部位繁殖，并侵入坐骨神经和脊髓;没有证据表明病毒在血液或内脏中繁殖。3.在老年小鼠中，肌肉注射多达1000万M.C.L.D.后，没有证据表明局部或系统性增殖;尽管存在数千例M.C.L.D.。病毒在接种部位至少4天后，坐骨神经或中枢神经系统没有可检测到的侵袭。4.将病毒直接注射到老年小鼠的坐骨神经中，导致半数动物出现典型的麻痹性疾病。5.在注射后3天内，病毒可在神经中被证实，但在脊髓或脑中未被证实。在麻痹发作时（第6天），在脊髓中可检测到病毒，但在接种的神经中不再检测到病毒。6.病毒从神经侵入中枢神经系统而不是从肌肉侵入的能力表明在肌肉或肌神经连接处存在屏障。7.将病毒注射到眼睛的玻璃体液中之后，在15日龄的小鼠中发生致命的脑炎，但是1岁的小鼠，除了少数例外，存活而没有显示疾病的迹象。8.在眼内注射的15日龄小鼠的脑中，病毒传播太快而不能指示所追求的途径，但在21日龄小鼠中，有证据表明主要途径可能是沿着视神经的轴突与对侧间脑和中脑交叉，随后早期传播到相应的枕叶皮质。然而，在有抵抗力的老年小鼠中，大脑的任何部分都没有发现病毒。
1. Injection of vesicular stomatitis virus into the leg muscles of young mice gives rise to flaccid paralysis of the inoculated extremity as the first clinical sign of a disease which is invariably fatal; old mice similarly injected with the largest doses of virus survive without exhibiting any signs of illness. 2. In young mice the virus was shown to multiply at the site of inoculation and to invade the sciatic nerve and spinal cord; there was no evidence of multiplication of virus in the blood or viscera. 3. In old mice, after intramuscular injection of as much as 10 million M.C.L.D., there was no evidence of either local or systemic multiplication; in spite of the persistence of thousands of M.C.L.D. of virus at the site of inoculation for at least 4 days, there was no detectable invasion of the sciatic nerve or the central nervous system. 4. Injection of the virus directly into the sciatic nerve of old mice led to the typical paralytic disease in half the number of animals. 5. For 3 days after intrasciatic injection the virus could be demonstrated in the nerve but not in the spinal cord or brain. At the onset of paralysis (6th day) virus was detectable in the spinal cord but no longer in the inoculated nerve. 6. The capacity of the virus to invade the central nervous system from the nerve but not from the muscle suggested the existence of a barrier in the muscle or myoneural junction. 7. Injection of the virus into the vitreous humor of the eye is followed by a fatal encephalitis in 15 day old mice, but 1 year old mice, with few exceptions, survive without showing signs of disease. 8. The spread of virus in the brains of intraocularly injected, 15 day old mice was too rapid to indicate the pathways which were pursued, but in 21 day old mice there was evidence that the primary pathway was probably along the axons of the optic nerve with decussation to the contralateral diencephalon and mesencephalon, and subsequent early spread to the corresponding occipital cortex. In resistant, old mice, however, no virus was found in any part of the brain.