Distribution of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in human tissues: Cellular surface localization of HAI-1 in simple columnar epithelium and its modulated expression in injured and regenerative tissues
Distribution of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in human tissues: Cellular surface localization of HAI-1 in simple columnar epithelium and its modulated expression in injured and regenerative tissues
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DOI：
10.1177/002215549904700509
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发表时间：
1999-05-01
影响因子：
3.2
通讯作者：
Koono, M
中科院分区：
文献类型：
--
作者：
Kataoka, H;Suganuma, T;Koono, M
我们使用了一种特异性的单克隆抗体，人肝细胞生长因子激活物抑制剂1型（HAI-1）的免疫组织化学方法，以确定HAI-1在人体组织中的分布和定位。在正常成人组织中，HAI-1主要表达于各种器官的导管、小管和粘膜表面的单层柱状上皮。在所有情况下，HAI-1主要定位于细胞外侧（或基底外侧）表面。与此相反，肝细胞，腺泡细胞，内分泌细胞，间充质细胞，和炎症细胞几乎不与抗体，复层鳞状上皮细胞的基底层细胞的表面上只表现出微弱的免疫反应。在胃肠道中，表面上皮被强烈染色。RNA印迹分析证实胃肠道粘膜中存在特异性mRNA转录物，原位杂交显示HAI-1 mRNA显示类似的细胞分布模式。虽然HAI-1在正常肝细胞中不表达，但在假胆管上皮和暴发性肝炎散在肝细胞表面观察到强免疫反应性。在梗死后的肾脏再生小管上皮细胞中也观察到表达增强。我们的结论是，HAI-1优先表达在粘膜表面和导管的单层柱状上皮，HAI-1的主要定位是细胞表面，并且HAI-1的表达可以通过组织损伤和再生来调节。
We used a specific monoclonal antibody to human hepatocyte growth factor activator inhibitor type 1 (HAI-1) in immunohistochemical procedures to determine the distribution and localization of HAI-1 in human tissues. In normal adult tissues, HAI-1 was predominantly expressed in the simple columnar epithelium of the ducts, tubules, and mucosal surface of various organs. In all cases, HAI-1 was localized predominantly on the cellular lateral (or basolateral) surface. By contrast, hepatocytes, acinar cells, endocrine cells, stromal mesenchymal cells, and inflammatory cells were hardly stainable with the antibody, and stratified squamous epithelium showed only faint immunoreactivity on the surface of cells of the basal layer. In the gastrointestinal tract, the surface epithelium was strongly stained. RNA blot analysis confirmed the presence of specific mRNA transcript in the gastrointestinal mucosa, and in situ hybridization revealed that HAI-1 mRNA showed a similar cellular distribution pattern. Although HAI-1 was not expressed in normal hepatocytes, strong immunoreactivity was observed on the epithelium of pseudo-bile ducts and on the surface of scattered hepatocytes in fulminant hepatitis. The enhanced expression was also noted in regenerating tubule epithelial cells of the kidney after infarction. We conclude that HAI-1 is preferentially expressed in the simple columnar epithelium of the mucosal surface and duct, that the predominant localization of HAI-1 is the cell surface, and that the expression of HAI-1 can be modulated by tissue injury and regeneration.