Roles of bta-miR-29b promoter regions DNA methylation in regulating miR-29b expression and bovine viral diarrhea virus NADL replication in MDBK cells
Roles of bta-miR-29b promoter regions DNA methylation in regulating miR-29b expression and bovine viral diarrhea virus NADL replication in MDBK cells
复制标题
bta-miR-29b启动子区DNA甲基化在调节MDBK细胞中miR-29b表达和牛病毒性腹泻病毒NADL复制中的作用
DOI：
10.1007/s00705-016-3107-1
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发表时间：
2017-02-01
影响因子：
2.7
通讯作者：
Chen, Chuangfu
中科院分区：
文献类型：
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作者：
Fu, Qiang;Shi, Huijun;Chen, Chuangfu
MicroRNA（miRNAs）是一类重要的非编码小RNA，在许多生物过程和细胞途径中通过降解靶mRNA或抑制蛋白质翻译来控制靶基因的表达。在先前的研究中，我们发现miR-29 b干扰牛病毒性腹泻病毒（BVDV）的复制。然而，miR-29 b表达的调控机制尚不清楚。DNA甲基化是基因转录沉默的重要表观遗传学机制，在启动子选择、蛋白质表达和miRNAs表达调控中起重要作用。本研究旨在探讨miR-29 b启动子DNA甲基化在miR-29 b表达调控中的作用，并研究DNMT 1基因敲低对miR-29 b表达和BVDV（NADL株）复制的影响。我们的结果表明，在BVDV NADL感染的MDBK细胞中，miR-29 b启动子的甲基化水平显著降低。DNMT 1沉默显著降低miR-29 b启动子甲基化水平，上调miR-29 b表达，抑制BVDV NADL复制，支持DNA甲基化在调控miRNA表达中的重要作用，进一步证实了我们之前的观点。
MicroRNAs (miRNAs) are an important class of small, non-coding RNAs that control target genes expression by degradation of target mRNAs or by inhibiting protein translation in many biological processes and cellular pathways. In a previous study, we found that miR-29b interfered with bovine viral diarrhea virus (BVDV) replication. However, the mechanisms of regulation of miR-29b expression are not well known. DNA methylation is an important epigenetic mechanism for silencing gene transcription, and plays an important role in promoter choice, protein expression, and regulation of miRNAs expression. In this study, we focused on the roles of DNA methylation of miR-29b promoter in regulating miR-29b expression and investigated the effects of DNA (cytosine-5) methyltransferase 1 (DNMT1) knockdown on miR-29b expression and BVDV (strain NADL) replication. Our results showed that methylation levels of miR-29b promoter were significantly decreased in BVDV NADL-infected MDBK cells. Furthermore, DNMT1 silencing significantly decreased the methylation levels of miR-29b promoter, up-regulated miR-29b expression and inhibited BVDV NADL replication, which supports the important roles of DNA methylation in regulating miRNA expression and further proves an evidence for our previous views.