Identification of crude drugs in the Japanese pharmacopoeia using a DNA barcoding system.
Identification of crude drugs in the Japanese pharmacopoeia using a DNA barcoding system.
复制标题
利用DNA条形码系统鉴定日本药典中的生药
DOI：
10.1038/srep42325
复制
发表时间：
2017-02-10
影响因子：
4.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Chen X;Xiang L;Shi L;Li G;Yao H;Han J;Lin Y;Song J;Chen S
汉布是日本传统草药的总称，被公认为官方药物，并列入日本药典（JP）。在大多数情况下，仅用传统的鉴定方法很难将原料药鉴定到种的水平。我们报告了第一个在线DNA条形码识别系统，其中包括大约95%的物种在JP（16版）中记录的标准条形码序列。该工具为用户提供了JP中记录的每种生药的基本信息，草药材料的DNA条形码鉴定，以及从取样到数据分析的标准操作程序（SOP）。从总共576份样本中生成ITS2序列（当ITS2不能扩增时，psba - trnhh是另一种选择）以建立数据库。使用该系统鉴定了另外100个样品（来自不同的药用部位，来自单一来源和多种来源，以及来自零售商和种植基地）。共有78%的测试样本被鉴定为其标签上所列的物种。该系统为中国、韩国、美国和欧盟等国家的药典建立了安全有效利用传统中草药的示范平台。
Kampo is the general designation for traditional Japanese herbal medicines, which are recognized as official medicines and listed in the Japanese pharmacopoeia (JP). In most cases, it is difficult to identify the crude drug materials to species level using only traditional identification methods. We report the first online DNA barcode identification system, which includes standard barcode sequences from approximately 95% of the species recorded in the JP (16thedition). This tool provides users with basic information on each crude drug recorded in the JP, DNA barcoding identification of herbal material, and the standard operating procedure (SOP) from sampling to data analysis. ITS2 sequences (psbA-trnHwas an alternative when ITS2 could not be amplified) were generated from a total of 576 samples to establish the database. An additional 100 samples (from different medicinal parts, from both single origin and multiple origins and from both retailers and the planting base) were identified using the system. A total of 78% of the test samples were identified as the species listed on their label. This system establishes a model platform for other pharmacopeias from countries like China, Korea, the US and the European Union, for the safe and effective utilization of traditional herbal medicines.