Effect of ciguatoxin 3C on voltage-gated Na+ and K+ currents in mouse taste cells
Effect of ciguatoxin 3C on voltage-gated Na+ and K+ currents in mouse taste cells
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DOI：
10.1093/chemse/bjl008
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发表时间：
2006-09-01
期刊：
影响因子：
3.5
通讯作者：
Bigiani, Albertino
中科院分区：
文献类型：
--
作者：
Ghiaroni, Valeria;Fuwa, Haruhiko;Bigiani, Albertino
海洋甲藻 Gambierdiscus 有毒产生高亲脂性多环醚毒素，导致海鲜中毒，称为雪卡毒素。雪卡毒素 (CTX) 和甘比醇是雪卡中毒的两种主要致病因子，其中包括味觉改变（味觉障碍）。然而，关于雪卡毒素在味觉细胞中的作用方式的信息很少。在这里，我们研究了合成 CTX3C（CTX 同系物）对小鼠味觉细胞的影响。通过使用膜片钳技术监测膜离子电流，我们发现CTX3C显着影响电压门控Na+通道的操作，但对电压门控K+通道无效。这个结果与我们之前用甘比尔罗获得的结果完全相反，甘比尔罗抑制 K+ 通道，但不抑制 Na+ 通道。因此，CTX 和甘比尔醇高效地影响味觉细胞中不同类别的电压门控离子通道的运行。我们的数据表明，雪卡中毒中报告的味觉障碍可能是由于雪卡毒素干扰味蕾离子通道的能力造成的。
The marine dinoflagellate Gambierdiscus toxicus produces highly lipophilic, polycyclic ether toxins that cause a seafood poisoning called ciguatera. Ciguatoxins (CTXs) and gambierol represent the two major causative agents of ciguatera intoxication, which include taste alterations (dysgeusiae). However, information on the mode of action of ciguatera toxins in taste cells is scarce. Here, we have studied the effect of synthetic CTX3C (a CTX congener) on mouse taste cells. By using the patch-clamp technique to monitor membrane ion currents, we found that CTX3C markedly affected the operation of voltage-gated Na+ channels but was ineffective on voltage-gated K+ channels. This result was the exact opposite of what we obtained earlier with gambierol, which inhibits K+ channels but not Na+ channels. Thus, CTXs and gambierol affect with high potency the operation of separate classes of voltage-gated ion channels in taste cells. Our data suggest that taste disturbances reported in ciguatera poisoning might be due to the ability of ciguatera toxins to interfere with ion channels in taste buds.