Further investigation of capacitation of sperm and fertilization of mouse eggs in vitro.
Further investigation of capacitation of sperm and fertilization of mouse eggs in vitro.
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精子获能和小鼠卵子体外受精的进一步研究。
DOI：
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发表时间：
1970
期刊：
影响因子：
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通讯作者：
M. Chang
中科院分区：
文献类型：
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作者：
T. Iwamatsu;M. Chang
在加热的牛卵泡液中，用新鲜附睾精子对注射HCG后14-16小时从输卵管中获得的小鼠卵子进行体外授精。在授精后1 ~ 8小时检查时，无顶体精子的比例增加，精子穿透卵子的比例从2%增加到79%，受精卵的比例从授精后2小时的13%增加到授精后8小时的67%。虽然穿透到每个卵子的精子的平均数量逐渐增加，直到受精后5小时，进入卵黄的精子数量在此期间没有增加，表明卵黄膜中存在阻滞机制。当新鲜附睾精子在牛卵泡液中预孵育3 ~ 4小时后用于授精时，精子穿透发生在20分钟后，精子穿透卵子的比例从20分钟时的17%增加到授精后2小时的90%，表明小鼠精子需要获能。当卵子与预孵育的精子授精时，精子穿透似乎发生得更快，并且无卵泡细胞的卵子中多精卵子的比例（17-35%）高于有卵泡细胞的卵子（3-23%）。
Mouse eggs obtained from oviducts 14–16 hours after an injection of HCG were inseminated in vitro with fresh epididymal sperm in the presence of heated bovine follicular fluid. When examined one to eight hours later the proportion of sperm without acrosome increased, the proportion of eggs penetrated by sperm increased from 2 to 79%, and that of eggs undergoing fertilization increased from 13% at two hours to 67% at eight hours after insemination. Although the average number of sperm that had penetrated into each egg increased progressively until five hours after insemination, the number of sperm that had entered the vitellus did not increase during this time, showing the existence of a block mechanism in the vitelline membrane. When fresh epididymal sperm were pre-incubated in the presence of bovine follicular fluid for three to four hours and used for insemination, sperm penetration occurred 20 minutes later and the proportion of eggs penetrated by sperm increased from 17% at 20 minutes to 90% at two hours after insemination, showing a requirement of capacitation of mouse sperm. When eggs were inseminated with pre-incubated sperm, sperm penetration appeared to occur sooner and the proportion of polyspermic eggs was higher in the eggs without follicular cells (17–35%) than in the eggs with follicular cells (3–23%).