Bcl-2 in LPS- and allergen-induced hyperplastic mucous cells in airway epithelia of Brown Norway rats.
Bcl-2 in LPS- and allergen-induced hyperplastic mucous cells in airway epithelia of Brown Norway rats.
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Bcl-2 在 LPS 和过敏原诱导的布朗挪威大鼠气道上皮增生粘液细胞中。
DOI：
10.1152/ajplung.2000.279.6.l1210
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Seagrave,J
中科院分区：
文献类型：
--
作者：
Tesfaigzi,Y;Fischer,MJ;Martin,AJ;Seagrave,J
环境毒素、感染和过敏原导致气道上皮短暂性粘液细胞增生（MCH）；然而，在恢复过程中减少粘液细胞数量的机制在很大程度上尚不清楚。本研究调查了中性粒细胞或嗜酸性粒细胞炎症反应诱导的粘液细胞中 Bcl-2 的表达。气管内滴注脂多糖（LPS）的棕色挪威大鼠表现出主要以中性粒细胞为特征的炎症反应。与未滴注的大鼠相比，在滴注 LPS 后第 1 天，分泌的粘蛋白增加了四倍，并且在滴注 LPS 后第 2、3 和 4 天，粘液细胞的数量增加了五倍。未注射或注射生理盐水的对照动物中，没有粘液细胞表达 Bcl-2，而在注射 LPS 后 1 天和 2 天，20-30% 的粘液细胞呈 Bcl-2 阳性。用卵清蛋白 (OVA) 免疫并攻击 2、4 和 6 天的挪威棕色大鼠显示出主要以嗜酸性粒细胞为特征的炎症反应。与接受 OVA 气雾剂攻击的水免疫对照大鼠相比，在 OVA 暴露 4 天和 6 天后，分泌的粘蛋白增加了五倍，粘液细胞数量增加了五倍。在 OVA 免疫和攻击的大鼠中，大约 10-25% 的粘液细胞呈 Bcl-2 阳性。这些数据表明，无论炎症反应的类型如何，棕色挪威大鼠的增生性粘液细胞中都有 Bcl-2 表达，并表明细胞凋亡机制可能与 MCH 的消退有关。
Environmental toxins, infection, and allergens lead to a transient mucous cell hyperplasia (MCH) in airway epithelia; however, the mechanisms for reducing mucous cell numbers during recovery are largely unknown. This study investigated Bcl-2 expression in mucous cells induced by a neutrophilic or eosinophilic inflammatory response. Brown Norway rats intratracheally instilled with lipopolysaccharide (LPS) showed an inflammatory response characterized primarily by neutrophils. Secreted mucin was increased fourfold at 1 day, and the number of mucous cells was increased fivefold 2, 3, and 4 days post-LPS instillation compared with those in noninstilled rats. None of the mucous cells in non- or saline-instilled control animals expressed Bcl-2, whereas 20–30% of mucous cells were Bcl-2 positive 1 and 2 days post-LPS instillation. Brown Norway rats immunized and challenged with ovalbumin (OVA) for 2, 4, and 6 days showed an inflammatory response characterized primarily by eosinophils. Secreted mucin increased fivefold, and mucous cell number increased fivefold after 4 and 6 days of OVA exposure compared with water-immunized control rats challenged with OVA aerosols. Approximately 10–25% of mucous cells were Bcl-2 positive in OVA-immunized and -challenged rats. These data demonstrate Bcl-2 expression in hyperplastic mucous cells of Brown Norway rats regardless of the type of inflammatory response and indicate that apoptotic mechanisms may be involved in the resolution of MCHs.