Effects of UV irradiation on the molecular weight of spider silk
Effects of UV irradiation on the molecular weight of spider silk
复制标题
紫外辐射对蜘蛛丝分子量的影响
DOI：
10.1038/pj.2013.41
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发表时间：
2013
期刊：
影响因子：
2.8
通讯作者：
Keizo Yamamoto and Shigeyoshi Osaki
中科院分区：
文献类型：
--
作者：
Takashi Matsuhira;Keizo Yamamoto and Shigeyoshi Osaki
使用SDS-PAGE方法测定棒新妇蜘蛛拖丝的分子量为272±14 kDa。研究了紫外线（UV）照射对蜘蛛丝分子量的影响，并与蚕丝的分子量进行了比较。使用 SDS-PAGE 观察到的蜘蛛拖丝和蚕丝的条带密度随着紫外线照射时间的延长而降低，表明由于蛋白质链分子的裂解而分解。结果发现，蚕丝因紫外线照射而导致的降解速率常数比棒棒猪笼草蜘蛛拉丝的降解速率常数大1.7。这一结果表明，N. clavata蜘蛛拉丝对紫外线照射的抵抗力是蚕丝的1.7倍。
The molecular weight of the Nephila clavata spider dragline was determined to be 272±14 kDa using a SDS-PAGE method. The effects of ultraviolet (UV) irradiation on the molecular weight of the spider dragline were studied and compared with that of silkworm silk. The band densities observed using SDS-PAGE for the spider dragline and silkworm silk decreased with the UV irradiation time, suggesting decomposition due to the cleavage of protein chain molecules. It was found that the rate constant of degradation due to UV irradiation for the silkworm silk was larger by 1.7 than that for the N. clavata spider dragline. This result indicates that the N. clavata spider dragline was 1.7 times more resistant to UV irradiation than silkworm silk.