Dissimilar differentiation of mesenchymal stem cells from bone marrow, umbilical cord blood, and adipose tissue
Dissimilar differentiation of mesenchymal stem cells from bone marrow, umbilical cord blood, and adipose tissue
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DOI：
10.3181/0712-rm-356
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发表时间：
2008-07-01
影响因子：
3.2
通讯作者：
Correa, A.
中科院分区：
文献类型：
--
作者：
Rebelatto, C. K.;Aguiar, A. M.;Correa, A.
间充质干细胞(MSCs)被认为是一种新的基于细胞的治疗策略，如间充质来源的组织修复。MSCs很容易从成人组织中分离出来，而且不受伦理限制。然而，与MSC相关的文献在MSC分化潜能和分子标记方面是相互矛盾的。在这里，我们比较了从骨髓(M)、脐带血(UCB)和脂肪组织(AT)分离的MSCs。BM和AT的分离率均为100%，而脐带血的分离率仅为30%。来自这些组织的MSCs在形态和免疫表型上是相似的，尽管它们的分化不同。通过细胞化学分析，所有来源的MSCs向成骨细胞和软骨母细胞的分化相似。成脂分化结果显示，脐带血来源的MSCs比骨髓来源的MSCs和AT来源的干细胞(ADSCs)产生的脂质空泡少且小(任意分化值分别为245.57+/-943和243.89+/-145.52µm(2)/核)。BM来源的MSCs和ADSCs的单个脂滴所占的平均面积分别为7.37微米(2)和2.36微米(2)，这一发现表明BM来源的MSCs比处理的ADSC培养的成熟脂肪细胞更多。我们通过定量聚合酶链式反应分析FAPB4、ALP和II型胶原基因的表达，以分别确认成脂、成骨和成软骨分化。结果显示，这三种来源的软骨和成骨分化能力相似，但它们的成脂潜力不同。因此，为将来的临床应用预先确定最合适的MSC来源可能是至关重要的。
Mesenchymal stem cells (MSCs) have been investigated as promising candidates for use in new cell-based therapeutic strategies such as mesenchyme-derived tissue repair. MSCs are easily isolated from adult tissues and are not ethically restricted. MSC-related literature, however, is conflicting in relation to MSC differentiation potential and molecular markers. Here we compared MSCs isolated from bone marrow (M), umbilical cord blood (UCB), and adipose tissue (AT). The isolation efficiency for both BM and AT was 100%, but that from UCB was only 30%. MSCs from these tissues are morphologically and immunophenotypically similar although their differentiation diverges. Differentiation to osteoblasts and chondroblasts was similar among MSCs from all sources, as analyzed by cytochemistry. Adipogenic differentiation showed that UCB-derived MSCs produced few and small lipid vacuoles in contrast to those of BM-derived MSCs and AT-derived stem cells (ADSCs) (arbitrary differentiation values of 245.57 +/- 943 and 243.89 +/- 145.52 mu m(2) per nucleus, respectively). The mean area occupied by individual lipid droplets was 7.37 mu m(2) for BM-derived MSCs and 2.36 mu m(2) for ADSCs, a finding indicating more mature adipocytes in BM-derived MSCs than in treated cultures of ADSCs. We analyzed FAPB4, ALP, and type II collagen gene expression by quantitative polymerase chain reaction to confirm adipogenic, osteogenic, and chondrogenic differentiation, respectively. Results showed that all three sources presented a similar capacity for chondrogenic and osteogenic differentiation and they differed in their adipogenic potential. Therefore, it may be crucial to predetermine the most appropriate MSC source for future clinical applications.