LncRNA AB073614 promotes the proliferation and inhibits apoptosis of cervical cancer cells by repressing RBM5 (Retracted article. See vol. 24, pg. 7201, 2020)
LncRNA AB073614 promotes the proliferation and inhibits apoptosis of cervical cancer cells by repressing RBM5 (Retracted article. See vol. 24, pg. 7201, 2020)
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DOI：
10.26355/eurrev_201903_17382
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发表时间：
2019-03-01
影响因子：
3.3
通讯作者：
Chen, C.
中科院分区：
文献类型：
--
作者：
Guo, L-Y;Qin, C-F;Chen, C.
目的：最近的研究已经确定了长链非编码RNA（lncRNA）在癌症发展中的关键作用。子宫颈癌（Cervical Cancer，CC）是一种常见的妇科恶性肿瘤。本研究旨在确定lncRNA AB 073614在CC进展中的作用。患者和方法：通过定量真实的时间-聚合酶链反应（qRT-PCR）测定3个CC细胞系和48个配对CC样品中AB 073614的相对水平。通过体外细胞凋亡实验、克隆形成实验和增殖实验，探讨AB 073614对CC细胞行为的潜在调节作用。结果：AB 073614在大肠癌组织中的表达明显高于癌旁组织; AB 073614基因的敲低抑制CC细胞的增殖，但诱导凋亡。此外，RBM 5（RNA结合基序5）在体外敲低AB 073614后在CC细胞中上调。结论：AB 073614通过下调RBM 5的表达，促进CC细胞增殖，抑制细胞凋亡，为CC的治疗提供了新的思路。
OBJECTIVE: Recent studies have determined the crucial role of long noncoding RNAs (lncRNAs) in cancer development. Cervical cancer (CC) is a common type of fatal gynecological cancer worldwide. This study aims to identify the role of lncRNA AB073614 in the progression of CC.PATIENTS AND METHODS: Relative level of AB073614 in 3 CC cell lines and 48 paired CC samples was determined by the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The potential regulatory effects of AB073614 on the cellular behaviors of CC cells were explored through apoptosis assay, colony formation assay, and proliferation assay in vitro. The underlying mechanism of AB073614 in mediating the progression of CC was also conducted.RESULTS: The AB073614 expression was remarkably higher in CC tissues than that in adjacent tissues. The knockdown of AB073614 inhibited proliferation but induced apoptosis of CC cells. In addition, RBM5 (RNA binding motif 5) was upregulated in CC cells after knockdown of AB073614 in vitro. Furthermore, a negative correlation was seen between the expressions of RBM5 and AB073614 in CC tissues.CONCLUSIONS: AB073614 could promote proliferation and inhibit apoptosis of CC cells through downregulating RBM5, which may offer a new therapeutic intervention for CC patients.