Transcriptome Analysis of a Progeny of Somatic Hybrids of Cultivated Rice (Oryza sativa L.) and Wild Rice (Oryza meyeriana L.) With High Resistance to Bacterial Blight
Transcriptome Analysis of a Progeny of Somatic Hybrids of Cultivated Rice (Oryza sativa L.) and Wild Rice (Oryza meyeriana L.) With High Resistance to Bacterial Blight
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DOI：
10.1111/jph.12066
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发表时间：
2013-05
影响因子：
1.5
通讯作者：
Xuming Wang;Jie Zhou;Yong Yang;Feibo Yu;Juan Chen;Chulang Yu;Fang Wang;Ye Cheng;Chengqi Yan-Chengqi
中科院分区：
文献类型：
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作者：
Xuming Wang;Jie Zhou;Yong Yang;Feibo Yu;Juan Chen;Chulang Yu;Fang Wang;Ye Cheng;Chengqi Yan-Chengqi
在此之前，我们利用不对称体细胞杂交技术，成功地将美耶氏稻（Oryza meyeriana）的抗白叶枯病（BB）性状引入到水稻中。经过多年的育种，一个近等基因系被命名为Y73与复发亲本O. sativa L. ssp。粳稻的履历。对一种广谱BB病原菌水稻黄单胞菌（Xanthomonas oryzae pv）具有较高的抗性。oryzae。为了深入了解这些植物的高抗性表型，我们使用Affymetrix水稻基因芯片芯片检测了接种后24小时Y73叶片对BB感染的转录组反应。微阵列分析显示，与对照相比，接种后叶片转录组受到的影响较小，只有0.22%的基因受到差异调控。然而，一些有趣的基因，包括一个NAC结构域蛋白，一个启动子反应蛋白，参与泛素化，一个糖基转移酶基因，两个转录因子（TFs）和两个未知基因上调超过5倍。Xoo感染后，代谢和细胞成分、tf和防御信号的基因，如丝裂原激活的蛋白激酶级联，也被显著诱导。这项研究提供了水稻（Y73）对Xoo感染的初始反应的全基因组视图，并阐明了一些可能在抗病中起重要作用的基因。
Previously, we successfully introduced the bacterial blight (BB) resistance trait from Oryza meyeriana into Oryza sativa using asymmetric somatic hybridization with O. meyeriana as the donor species. After years of breeding, a near-isogenic line named Y73 was generated with recurrent parent O. sativa L. ssp. japonica cv. Dalixiang, and it shows high resistance to a broad-spectrum BB pathogens Xanthomonas oryzae pv. oryzae. To provide insights into the high-resistance phenotype of these plants, we examined the transcriptome response in leaves of Y73 to BB infection 24 h postinoculation using Affymetrix Rice GeneChip microarrays. Microarray analysis revealed that there was little impact on the transcriptome of inoculated leaves compared with controls, with only 0.22% of genes that were differentially regulated. However, several interesting genes, including a NAC domain–containing protein, an elicitor-responsive protein, involved in ubiquitination, and a glycosyl transferase gene, two transcription factors (TFs) and two unknown genes were up-regulated more than five-fold. Genes for metabolic and cellular components, TFs and defence signalling, such as the mitogen-activated protein kinase cascade, were also significantly induced after Xoo infection. This study provides a genome-wide view of the initial reaction of rice (Y73) to Xoo infection and elucidates some of the genes that may play an important role in disease resistance.