DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN DISEASED AND NONDISEASED SITES OF THE RESPIRATORY-TRACT IN RECURRENT RESPIRATORY PAPILLOMATOSIS PATIENTS BY DNA HYBRIDIZATION
DETECTION OF HUMAN PAPILLOMAVIRUS INFECTION IN DISEASED AND NONDISEASED SITES OF THE RESPIRATORY-TRACT IN RECURRENT RESPIRATORY PAPILLOMATOSIS PATIENTS BY DNA HYBRIDIZATION
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DOI：
10.1177/000348949210100506
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发表时间：
1992-05-01
影响因子：
1.4
通讯作者：
TUREK, LP
中科院分区：
文献类型：
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作者：
PIGNATARI, S;SMITH, EM;TUREK, LP
我们对 12 名正在接受疾病复发治疗的复发性呼吸道乳头状瘤病患者进行了检查，以确定是否存在人乳头瘤病毒 (HPV)。 从他们的呼吸道乳头状瘤和呼吸道非患病部位（NDS）获取活检：鼻咽、扁桃体后柱、杓会厌襞、颈部气管、胸内气管和支气管。 使用 ViraPap/ViraType DNA 杂交程序确定 HPV DNA 的存在。 三分之二的患者在患病乳头状瘤或 NDS 中感染 HPV 6/11：50% 的乳头状瘤标本患者分型呈阳性（12 例中的 6 例），40% 的患者在 NDS 的一项或多项活检中分型 HPV 阳性（10 例中的 4 例）。 没有任何一种 NDS 比其他任何一种 NDS 更容易感染 HPV。 在这些患者的乳头状瘤或 NDS 中未检测到致癌 HPV 16/18 或 31/33/35 型。 只有多发性而非孤立性乳头状瘤受累的患者在 NDS 中才发现含有 HPV DNA； 80% 感染 NDS 的患者需要更短（不超过 3 个月）的手术治疗间隔，而未感染 NDS 的患者只有 20%。 我们的结果表明，HPV 感染经常持续存在于邻近的临床正常部位，这表明 NDS 受累的程度可以预测疾病的程度和复发的可能性。
We examined 12 recurrent respiratory papillomatosis patients, who were undergoing treatment for recurrence of their disease, for the presence of human papillomavirus (HPV). Biopsies were obtained from their respiratory papillomas and nondiseased sites (NDS) of the respiratory tract: the nasopharynx, posterior tonsillar pillar, aryepiglottic fold, cervical trachea, intrathoracic trachea, and bronchi. The presence of HPV DNA was determined by using the ViraPap/ViraType DNA hybridization procedure. Two thirds of the patients were infected with HPV 6/11 in either the diseased papilloma or NDS: 50% of patients with papilloma specimens typed positive (6 of 12), and 40% of the patients typed HPV-positive in one or more biopsies from the NDS(4 of 10). No single NDS was more likely to be infected with HPV than any other. No oncogenic HPV types 16/18 or 31/33/35 were detected in the papillomas or NDS of these patients. Only patients with multiple, and not isolated, papilloma involvement were found to harbor HPV DNA in NDS; and 80% of those with infected NDS, compared to 20% of those without infected NDS, required a shorter (no more than 3 months) surgical treatment interval. Our results show that HPV infection frequently persists in adjacent, clinically normal sites, and suggest that the extent of NDS involvement may predict both the extent of disease and the likelihood of recurrence.