Statistical Estimate of the Total Number of Operons Specific for Bacillus subtilis Sporulation
Statistical Estimate of the Total Number of Operons Specific for Bacillus subtilis Sporulation
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枯草芽孢杆菌孢子形成特异操纵子总数的统计估计
DOI：
10.1128/jb.119.3.684-690.1974
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发表时间：
1974
影响因子：
3.2
通讯作者：
J. Mandelstam
中科院分区：
文献类型：
--
作者：
D. Hranueli;P. Piggot;J. Mandelstam
作为详尽作图的替代方法，已经尝试通过统计处理获得孢子形成操纵子总数的粗略估计。对随机抽取的16株产孢突变体进行了生化和形态特征分析。它们包含的突变被绘制出来，以确定它们是否属于23个已知操纵子中的任何一个。从这样做的比例(10/16)，计算出最可能的孢子形成操纵子的数量是37(68%的可信区间31和46)。如果考虑到其中两个操纵子显然含有诱变的“热点”这一事实，并且相应地修正了计算，则最可能的操纵子数目变成42个(限制33和59)。
As an alternative to exhaustive mapping, an attempt has been made to obtain a rough estimate of the total number of sporulation operons by statistical treatment. Sixteen sporulation mutants taken at random were characterized biochemically and morphologically. The mutations they contained were mapped to determine whether they fell into any one of 23 known operons. From the proportion that do so (10/16), it is calculated that the most probable number of sporulation operons is 37 (68% confidence limits of 31 and 46). If allowance is made for the fact that two of the operons apparently contain mutagenic “hot spots” and the calculation is amended accordingly, the most probable numbers of operons becomes 42 (limits 33 and 59).