Preparation and properties of nano-hydroxyapatite/PCL-PEG-PCL composite membranes for tissue engineering applications
Preparation and properties of nano-hydroxyapatite/PCL-PEG-PCL composite membranes for tissue engineering applications
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DOI：
10.1002/jbm.b.31788
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发表时间：
2011-04-01
影响因子：
3.4
通讯作者：
Qian, Zhi Yong
中科院分区：
文献类型：
--
作者：
Fu, Shao Zhi;Wang, Xiu Hong;Qian, Zhi Yong
采用溶剂铸造和蒸发法制备了纳米羟基磷灰石(n-HA)/聚（epsilon-己内酯）-聚（epsilon-己内酯）（PCL-PEG-PCL， PCEC）复合膜。采用傅里叶变换红外光谱（FT-IR）、x射线衍射（XRD）、扫描电镜（SEM）、水接触角测量、体外水解降解、力学性能测试和细胞培养等方法对膜的结构和性能进行了研究。研究了n-HA含量对n-HA/PCEC复合膜理化性能的影响。结果表明：复合膜微孔的形状和大小随n-HA含量的增加而变化；拉伸强度随n-HA含量的增加而降低。在膜上培养成骨细胞，培养1 d后观察到良好的细胞附着和生长方式。MTT实验表明，n-HA/PCEC膜对细胞活力和增殖没有负面影响。这些结果表明，本研究获得的n-HA/PCEC复合膜在组织工程领域具有广阔的应用前景。(C) 2011 Wiley期刊公司[J]中国生物医学工程学报，2011,31(2):557 - 557。
Nano-hydroxyapatite (n-HA)/poly(epsilon-caprolactone)poly(ethylene glycol)-poly(epsilon-caprolactone) (PCL-PEG-PCL, PCEC) composite membranes were prepared by solvent casting and evaporation method. The structure and properties of the membranes were investigated by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), water contact angle measurements, in vitro hydrolytic degradation, mechanical test, and cell culture. The effect of n-HA content on physical-chemical properties of the n-HA/PCEC composite membranes was studied. The results showed that the shape and size of micropores of the composite membranes changed with n-HA content increased; the tensile strength decreased with the increase of n-HA content. The osteoblast cell was cultured on the membranes, good cell attachment and growth manner were observed after postseeding for 1 day. MTT assays showed that the n-HA/PCEC membranes had no negative effect on the cell viability and proliferation. These results suggested that the obtained n-HA/PCEC composite membranes in this study might have prospective applications in tissue engineering field. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 97B: 74-83, 2011.