Accumulation of aluminium in the cell wall pectin in cultured tobacco (Nicotiana tabacum L.) cells treated with a combination of aluminium and iron
Accumulation of aluminium in the cell wall pectin in cultured tobacco (Nicotiana tabacum L.) cells treated with a combination of aluminium and iron
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DOI：
10.1046/j.1365-3040.1999.00467.x
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发表时间：
1999-08-01
影响因子：
7.3
通讯作者：
Matsumoto, H
中科院分区：
文献类型：
--
作者：
Chang, YC;Yamamoto, Y;Matsumoto, H
在营养培养基中，铝（AI）在烟草细胞中的积累只发生在亚铁离子[Fe（II）]的存在下。铝处理的细胞用纤维素酶和果胶裂解酶共同消化后，所得到的原生质球含有与完整细胞一样多的铝。然而，从铝处理的细胞中分离的细胞壁也含有与完整细胞一样多的铝。从完整细胞和原生质球分离的细胞壁化学提取的多糖组分中的糖和铝含量的比较表明，酶消化的大部分纤维素和半纤维素，但只有一半的果胶，和铝主要存在于果胶留在原生质球。从完整细胞的细胞壁的果胶级分（NH 4-草酸盐提取物）的凝胶渗透色谱法表明，铝与约3-7 kDa的小多糖。这些结果表明，果胶的一小部分是铝积累的主要网站。营养液中铝处理使细胞壁果胶含量增加。综上所述，我们假设Al mag与Al处理期间新产生的果胶结合。
In nutrient medium, aluminium (AI) accumulation in tobacco cells occurs only in the presence of ferrous ion [Fe(II)]. The localization of Al was examined to elucidate a mechanism of Al accumulation After the digestion of Al-treated cells with cellulase and pectolyase together, the resulting spheroplasts contained as much Al as the intact cells. However, the cell walls isolated from Al-treated cells also contained as much Al as the intact cells. Comparison of sugar and Al contents in polysaccharide components extracted chemically from cell walls isolated from intact cells and spheroplasts revealed that the enzymes digested most of the cellulose and hemicellulose, but only half of the pectin, and that Al mainly existed in the pectin remaining in the spheroplasts. Gel-permeation chromatography of the pectin fraction (NH4-oxalate extract) from the cell walls of the intact cells indicated that Al was associated with small polysaccharides of approximately 3-7 kDa. These results suggest that a minor part of pectin is a major site of Al accumulation. The content of cell wall pectin increased during Al treatment in nutrient medium. Taken together, we hypothesize that Al mag bind to the pectin newly produced during Al treatment.