Compositional and molecular species analysis of phospholipids by high performance liquid chromatography coupled with chemical ionization mass spectrometry.
Compositional and molecular species analysis of phospholipids by high performance liquid chromatography coupled with chemical ionization mass spectrometry.
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DOI：
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发表时间：
1984-07
影响因子：
6.5
通讯作者：
F. B. Jungalwala;J. Evans;R. McCluer
中科院分区：
文献类型：
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作者：
F. B. Jungalwala;J. Evans;R. McCluer
通过移动带接口将高效液相色谱（HPLC）与化学电离质谱（CIMS）联用。该技术用于分析天然存在的磷脂。正离子和负离子质谱的各种磷脂，如磷脂酰胆碱，磷脂酰乙醇胺，磷脂酰丝氨酸，磷脂酰肌醇，磷脂酰甘油，和鞘磷脂的化学电离模式与氨或甲烷作为试剂气体。个别磷脂“基地”的具体离子进行了鉴定。这些离子用于HPLC-CIMS期间磷脂的特定离子监测。每种磷脂的CIMS还提供了关于磷脂的单个类别的分子种类的广泛信息。通过CIMS测定的每种磷脂的不同分子种类的相对丰度与通过气液色谱法获得的结果一致。在约15分钟内通过HPLC-CIMS分析大鼠脑磷脂。然而，通过HPLC-CIMS分析了约5微克的单个磷脂，然而，通过特定离子监测，该方法提供了亚纳克水平的检测能力。
High performance liquid chromatography (HPLC) was combined with chemical ionization mass spectrometry (CIMS) by the use of a moving-belt interface. The technique was employed for the analysis of naturally occurring phospholipids. Positive and negative ion mass spectra of various phospholipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, and sphingomyelin were obtained in the chemical ionization mode with ammonia or methane as the reagent gas. Specific ions for individual phospholipid "bases" were identified. These ions were used in specific ion monitoring of the phospholipids during HPLC-CIMS. CIMS of each phospholipid also provided extensive information on the molecular species of the individual class of phospholipids. Relative abundance of different molecular species of each phospholipid as determined by CIMS agreed well with the results obtained by gas-liquid chromatography. Rat brain phospholipids were analyzed by HPLC-CIMS in about 15 minutes. Routinely, about 5 micrograms of individual phospholipid was analyzed by HPLC-CIMS, however, with specific ion monitoring the method provides a detection capability at the subnanogram level.