Alternative splicing and structure of the human and mouse SFRS5/HRS/SRp40 genes
Alternative splicing and structure of the human and mouse SFRS5/HRS/SRp40 genes
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DOI：
10.1016/s0378-1119(97)00552-0
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发表时间：
1997-12-19
期刊：
影响因子：
3.5
通讯作者：
Taub, R
中科院分区：
文献类型：
--
作者：
Du, KY;Taub, R
HRS/SRp 40/SFRS 5（HRS）是一种富含丝氨酸-精氨酸（serine-arginine-rich，SR）的蛋白质，可调节可变剪接和基础剪接。HRS mRNA包含几种转录本，包括HRS-SF和HRS-LF，它们在增殖的肝脏中具有不同的表达时间模式。如前所述，HRS-SF mRNA编码SR剪接因子。然而，HRS-LF的身份仍然不明。在此，我们克隆并鉴定了小鼠HRS基因、部分人HRS基因和来自HRS-LF mRNA的几个cDNA。小鼠HRS基因全长5050 bp，包含8个外显子和7个内含子，HRS-LF mRNA在SF mRNA内包含1.2 kb插入片段，终止密码子位于所有三个阅读框中。HRS-LF和HRS基因的比较表明，HRS-LF mRNA是一个含有内含子5的内含子保留产物。HRS-LF最多编码具有一个RNA结合结构域的截短HRS蛋白。有趣的是，内含子5在小鼠和人HRS基因之间表现出90%的同一性，这意味着内含子5可能在调节HRS基因剪接或表达中起重要作用。(C)1997年Elsevier Science B.V.
HRS/SRp40/SFRS5 (HRS) is an SR (serine-arginine-rich) protein which regulates both alternative splicing and basal splicing. HRS mRNA contains several transcripts, including HRS-SF and HRS-LF which have different temporal patterns of expression in proliferating liver. As previously reported, HRS-SF mRNA encodes the SR splicing factor. However, the identity of HRS-LF remained unknown. Here, we cloned and characterized the mouse HRS gene, partial human HRS gene, and several cDNAs derived from HRS-LF mRNA. The mouse HRS gene spans 5050 bp and contains eight exons and seven introns HRS-LF mRNA contains a 1.2 kb insert within the SF mRNA with stop codons in all three reading frames. A comparison of HRS-LF and the HRS gene revealed that HRS-LF mRNA is an intron-retaining product which contains intron 5. At most, HRS-LF encodes a truncated HRS protein with one RNA binding domain. Interestingly, intron 5 demonstrates 90% identity between the mouse and human HRS genes, implying that intron 5 might play an important role in regulating HRS gene splicing or expression. (C) 1997 Elsevier Science B.V.