Follistatin gene expression in the ovary and extragonadal tissues.
Follistatin gene expression in the ovary and extragonadal tissues.
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DOI：
10.1210/mend-3-4-651
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发表时间：
1989-04
影响因子：
--
通讯作者：
S. Shimasaki;M. Koga;M. Buscaglia;D. Simmons;T. Bicsak;N. Ling
中科院分区：
文献类型：
--
作者：
S. Shimasaki;M. Koga;M. Buscaglia;D. Simmons;T. Bicsak;N. Ling
卵泡抑素是一种糖基化的单链蛋白，最初从猪卵泡液中分离出来。它能特异性地抑制脑下垂体分泌FSH。我们现在已经从PMSG刺激的卵巢文库中分离并鉴定了大鼠卵泡抑素的cDNA。大鼠卵泡抑素前体推导的氨基酸序列与猪和人卵泡抑素高度同源(大于98%)，包括潜在的天冬氨酸糖基化位点。此外，还分离了大鼠卵泡抑素的基因组克隆，发现大鼠、猪和人的卵泡抑素基因结构的外显子和内含子组织结构是保守的。在大鼠组织中的Northern分析表明，卵泡抑素基因不仅在卵巢中表达，而且在肾脏和脑中也有表达。在未成熟大鼠卵巢中，注射PMSG(20IU/只)可刺激卵泡抑素mRNA的表达，但注射PMSG后不受人CG(10IU/只)的影响。原位杂交结果显示，在原始卵泡中，卵巢中的mRNA水平较低，而在发育中的次级卵泡和第三级卵泡中，颗粒细胞中的mRNA水平显著升高，而在成熟的排卵前卵泡中，其表达水平则下降。肾外髓集合管上有较强的卵泡抑素信使核糖核酸信号，脑内有弱至中度信号。卵泡抑素的广泛组织分布强烈提示，除了抑制脑垂体FSH的释放外，卵泡抑素还有其他生理作用。
Follistatin is a glycosylated single-chain protein originally isolated from porcine follicular fluid. It specifically inhibits the secretion of FSH from the pituitary. We have now isolated and characterized a cDNA for rat follistatin from the PMSG-stimulated ovarian library. The deduced amino acid sequence of the rat follistatin precursor is highly homologous (greater than 98%) to porcine and human follistatins including potential Asn-glycosylation sites. The genomic clone encoding rat follistatin was also isolated and revealed that the exon and intron organization of the follistatin gene structure is conserved among rat, porcine, and human. Northern analyses in rat tissues demonstrated that the follistatin gene is expressed not only in the ovary but also in the kidney and brain. In the immature rat ovary, the follistatin mRNA level is stimulated by PMSG injection (20 IU/rat), but is not affected by human CG (10 IU/rat) after PMSG administration. In situ hybridization studies revealed that the mRNA level in the ovary was low in primordial follicles, but dramatically increased in the granulosa cells of the growing secondary and tertiary follicles and then decreased in the mature preovulatory follicles. A strong follistatin mRNA signal was observed over the collecting tubules of the outer medulla of the kidney, and a weak to moderate signal was detected in brain. The broad tissue distribution of follistatin mRNA strongly suggests other physiological roles for follistatin besides the inhibition of pituitary FSH release.