UNCOUPLING OF OXIDATIVE PHOSPHORYLATION BY CARBONYL CYANIDE PHENYLHYDRAZONES .1. SOME CHARACTERISTICS OF M-CL-CCP ACTION ON MITOCHONDRIA AND CHLOROPLASTS
UNCOUPLING OF OXIDATIVE PHOSPHORYLATION BY CARBONYL CYANIDE PHENYLHYDRAZONES .1. SOME CHARACTERISTICS OF M-CL-CCP ACTION ON MITOCHONDRIA AND CHLOROPLASTS
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DOI：
10.1021/bi00902a031
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发表时间：
1963-01-01
期刊：
影响因子：
2.9
通讯作者：
HEYTLER, PG
中科院分区：
文献类型：
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作者：
HEYTLER, PG
间氯苯肼(m-Cl-CCP)是植物、动物和昆虫线粒体氧化磷酸化的有效解偶联剂，浓度在10-7~10-6M之间。虽然在某些方面它对线粒体的作用类似于2，4-二硝基苯酚[长划线]，例如-完整线粒体的表观ATPase被刺激，静止线粒体的膨胀被抑制[长划线]m-Cl-CCP也表现出几个不寻常的特征。它不会刺激或抑制纯化的可溶性物质。来自线粒体的ATPase；它的作用被1，2-和1，3-氨基硫醇特异性地阻断；它强烈地抑制叶绿体中的光磷酸化。M-氯化合物是CCP解偶联剂的代表，这类解偶联剂似乎是磷酸化研究中有用的工具。
Carbonyl cyanide m-chlorophenylhydrazone (m-Cl-CCP), at concentrations between 10-7 and 10-6 M, is an effective un-coupler of oxidative phosphorylation in plant, animal, and insect mitochondria. While in some respects its action on mitochondria resembles that of 2,4-dinitrophenol[long dash]e.g.- apparent ATPase of intact mitochondria is stimulated and swelling of resting mitochondria is inhibited[long dash]m-Cl-CCP also exhibits several unusual characteristics. It does not stimulate or inhibit purified soluble. ATPase from mitochondria; its action is specifically blocked by 1,2- and 1,3-aminothiols; and it strongly inhibits photophosphorylation in chloroplasts. The m-Cl compound is representative of the class of CCP uncouplers, which appear to be useful tools in phosphorylation studies.