Antioxidant regulatory mechanisms in neutrophils and lymphocytes after intense exercise
Antioxidant regulatory mechanisms in neutrophils and lymphocytes after intense exercise
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DOI：
10.1080/02640410802409683
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发表时间：
2009-01-01
影响因子：
3.4
通讯作者：
Pons, Antoni
中科院分区：
文献类型：
--
作者：
Ferrer, Miguel D.;Tauler, Pedro;Pons, Antoni
本研究的目的是评估游泳对外周血中性粒细胞和淋巴细胞的促抗氧化系统的影响，确定性别之间的差异以及可能诱导免疫细胞对运动的适应性反应的调节机制。24名游泳运动员（15名男性，9名女性）以75-80%的最大能力参加了一个小时的游泳训练。会议诱导嗜中性粒细胞和抗氧化酶活性和抗坏血酸水平下降。雄性和雌性中性粒细胞中丙二醛升高，而羰基指数仅在雄性中升高。基线时雄性动物的淋巴细胞谷胱甘肽过氧化物酶活性较高，运动后升高。运动降低解偶联蛋白-3和Bcl-2基因表达。过氧化物酶体增殖物激活物γ共激活因子-1 α（PGC-1 α）的表达与沉默调节蛋白3（SIRT 3）和过氧化氢酶的表达呈正相关。总之，以75-80%的最大能力游泳1小时，会导致中性粒细胞氧化损伤，并诱导淋巴细胞的抗氧化防御。PGC-1 α和SIRT 3似乎是淋巴细胞中这种适应性反应的关键效应子。中性粒细胞和淋巴细胞对运动的反应，女性略弱于男性。
The aims of this study were to assess the effects of a swimming session on the peripheral blood neutrophil and lymphocyte pro- and antioxidant system, identify any differences between the sexes and the regulatory mechanisms that might induce the immune cell adaptive response to exercise. Twenty-four swimmers (15 males, 9 females) participated in a one-hour swimming session at 75-80% of their maximal capacity. The session induced neutrophilia and decreased antioxidant enzyme activities and ascorbate levels in neutrophils. Malondialdehyde rose in neutrophils in males and females, whereas the carbonyl index only increased in males. Lymphocyte glutathione peroxidase activity was higher in males at baseline and rose as a consequence of exercise. The exercise decreased uncoupling protein-3 and Bcl-2 gene expression. The expression of PPAR gamma coactivator-1 alpha (PGC-1 alpha) correlated positively with that of sirtuin 3 (SIRT3) and catalase. In summary, a swimming session of one hour at 75-80% of maximal capacity produced oxidative damage in neutrophils and induced the antioxidant defences in lymphocytes. PGC-1 alpha and SIRT3 appear to be key effectors of this adaptive response in lymphocytes. Both the neutrophil and lymphocyte response to exercise were slightly weaker in females than males.