Analysis of simian virus 40 infection of CV-1 cells by quantitative two-color fluorescence with flow cytometry.
Analysis of simian virus 40 infection of CV-1 cells by quantitative two-color fluorescence with flow cytometry.
复制标题
通过流式细胞术定量双色荧光分析猿病毒 40 对 CV-1 细胞的感染。
DOI：
10.1002/cyto.990090109
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Fogleman,D
中科院分区：
文献类型：
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作者：
Lehman,JM;Laffin,J;Jacobberger,JW;Fogleman,D
对猴病毒(SV40)感染允许的CV-1细胞进行了双色荧光定量分析。分析包括用细胞DNA定量，用单抗测定早期病毒肿瘤(T)抗原，用多克隆抗体测定晚期病毒(V)抗原。SV40感染细胞后6h和12h，细胞周期各时相均可检测到T抗原。在以后的时间间隔，T抗原阳性细胞的百分比随着细胞进入连续的DNA合成周期和四倍体多倍体细胞的增加而增加。随着细胞进入细胞周期的连续阶段(G0/G1、→、G2+M、→四倍体、S和G2+M)，每个细胞的T抗原量增加。感染后即可检测到吸附病毒的V抗原。V抗原的合成始于细胞周期的S晚期和G2+M期。这种定量分析可以确定与细胞周期相关的群体中每个细胞的抗原，并可能有助于将病毒和细胞事件与转化联系起来。
Quantitative two‐color fluorescent analysis of Simian virus (SV40) infection of permissive CV‐1 cells was investigated. Analysis included by quantitation of cellular DNA, the early viral tumor (T) antigen with a monoclonal antibody, and late viral (V) antigens with a polyclonal antibody. T antigen was detected in all phases of the cell cycle at 6 and 12 h, after SV40 infection of growth arrested cells. At later time intervals, the percentage of T‐antigen‐positive cells increased with the induction of the cells into successive rounds of DNA synthesis and an increase in tetraploidpolyploid cells. The amount of T antigen per cell increased as the cells entered the successive stages of the cell cycle (G0/G1→ G2+ M → tetraploid S and G2+ M). The V antigen from adsorbed virus was detected immediately after infection. Synthesis of V antigen began in late S and G2+ M phases of the cell cycle. This quantitative analysis allows a definitive determination of antigen per cell in a population correlated with the cell cycle and may be useful in correlating viral and cellular events with transformation.