Phylogenetic analysis of rabbit haemorrhagic disease virus in France between 1993 and 2000, and the characterisation RHDV antigenic variants
Phylogenetic analysis of rabbit haemorrhagic disease virus in France between 1993 and 2000, and the characterisation RHDV antigenic variants
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DOI：
10.1007/s00705-002-0908-1
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发表时间：
2003-01-01
影响因子：
2.7
通讯作者：
Rasschaert, D
中科院分区：
文献类型：
--
作者：
Le Gall-Reculé, G;Zwingelstein, F;Rasschaert, D
1988年至1995年在法国进行的第一次兔出血症病毒分子流行病学研究确定了三个基因组，其中两个（G1，G2）很快消失。我们使用免疫捕获-RT-PCR和测序分析104个新的RHDV分离株之间收集的1993年和2000年。2000年从法国海外领土留尼汪岛获得一个分离株。这些分离株的核苷酸序列与先前鉴定的代表三个基因组的一些法国RHDV分离株、一些参考株以及德国和美国RHDV抗原变体的核苷酸序列进行比对。尽管核苷酸序列变异程度较低，但三个新的基因组（G4至G6）被确定为具有显著的自举值。其中两个基因组（G4和G5）与RHDV分离株收集的年份有关。基因组G4来自基因组G3，现在已经消失了。G5基因组是一个新的独立基因组。基因组G6包含1999年在法国大陆收集的分离株和从留尼汪岛收集的分离株，以及德国和美国的RHDV变体。VP 60基因的多重序列比对和单克隆抗体的抗原性分析表明，这些法国分离株是RHDV变异株的两个新分离株。
The first molecular epidemiological study of Rabbit haemorrhagic disease virus undertaken in France between 1988 and 1995, identified three genogroups, two of which (G1, G2) disappeared quickly. We used immunocapture-RT-PCR and sequencing to analyse 104 new RHDV isolates collected between 1993 and 2000. One isolate was obtained in 2000 from a French overseas territory, the Reunion Island. The nucleotide sequences of these isolates were aligned with those of some French RHDV isolates representative of the three genogroups previously identified, of some reference strains and German and American RHDV antigenic variants. Despite the low degree of nucleotide sequence variation, three new genogroups (G4 to G6) were identified with significant bootstrap values. Two of these genogroups (G4 and G5) were related to the year in which the RHDV isolates were collected. Genogroup G4 emerged from genogroup G3, which has now disappeared. Genogroup G5 is a new independent group. The genogroup G6 contained an isolate collected in mainland France in 1999 and the isolate collected from the Reunion Island, as well as German and American RHDV variants. Multiple sequence alignments of the VP60 gene and antigenic analysis with monoclonal antibodies demonstrated that these French isolates are two new isolates of the RHDV variant.