Expression profile of microRNAs in expressed prostatic secretion of healthy men and patients with IIIA chronic prostatitis/chronic pelvic pain syndrome.
Expression profile of microRNAs in expressed prostatic secretion of healthy men and patients with IIIA chronic prostatitis/chronic pelvic pain syndrome.
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DOI：
10.18632/oncotarget.24069
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发表时间：
2018-02-23
期刊：
影响因子：
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通讯作者：
Lan T
中科院分区：
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作者：
Chen Y;Chen S;Zhang J;Wang Y;Jia Z;Zhang X;Han X;Guo X;Sun X;Shao C;Wang J;Lan T
本研究旨在确定健康男性和慢性前列腺炎/慢性盆腔疼痛综合征(CP/CPPS)患者前列腺液(EPS)中microRNAs(MiRNAs)的全面表达谱。在对382名参与者进行临床筛选后，从2012年3月至2014年12月，60名健康男性和59名有明显盆腔疼痛的IIIA CP/CPPS患者纳入本研究。采用高通量测序方法鉴定EPS-miRNAs的特征表达谱。QRT-PCR进一步证实差异EPS-miRNAs水平升高。最后，对21例随访患者的候选EPS-miRNAs进行跟踪检测，并用ROC曲线分析其对IIIA CP/CPPS分类的准确性。在发现噬菌体中，从40名健康男性和39名IIIA CP/CPPS患者的EPS混合样本中分别发现41和43个EPS-miRNAs。此外，22个丰富的EPS-miRNA在20名患者中被≥上调了2倍，而在20名健康男性中则是2倍。在噬菌体检测中，33例患者进一步证实miR-21-5p、miR-30a-5p、miR-30d-5p、miR-103a-3p和miR-141-3p水平升高，并与30名健康男性对照。在验证噬菌体实验中，21例随访患者的盆腔疼痛症状缓解伴随着miR-21-5p、miR-103a-3p和miR-141-3p的显著下调。ROC曲线分析表明，MIR-21-5P的ROC曲线下面积最大(0.891)，其次是MIR-141-3P和MIR-103A-3P。我们的研究为EPS中存在分泌型miRNAs提供了证据，EPS-miRNAs异常与前列腺炎有关。特别是miR-21-5p对有明显盆腔疼痛的IIIA CP/CPPS患者具有较高的分类准确性。
The current study aimed to identify a comprehensive expression-profile of microRNAs (miRNAs) in expressed prostatic secretion (EPS) collected from healthy men and patients with CP/CPPS (Chronic prostatitis/Chronic pelvic pain syndrome). After clinical screening of 382 participants, 60 healthy men and 59 IIIA CP/CPPS patients with significant pelvic-pain were included into this study from March 2012 to December 2014. High-throughput sequencing was employed to identify characteristic expression-profile of EPS-miRNAs. QRT-PCR was further performed to confirm elevated levels of differential EPS-miRNAs. Finally, candidate EPS-miRNAs were measured traceably in 21 follow-up patients and their classify-accuracy on IIIA CP/CPPS were analyzed by ROC (receiver operating characteristic) curve. In discovery-phage, 41 and 43 predominant EPS-miRNAs were found in pooled EPS-sample from 40 healthy men and 39 IIIA CP/CPPS patients, respectively. Furthermore, 22 abundant EPS-miRNAs were up-regulated with ≥ 2-fold in 20 patients compared to 20 healthy men. In testing-phage, elevated levels of miR-21-5p, miR-30a-5p, miR-30d-5p, miR-103a-3p and miR-141-3p were further confirmed in 33 patients by comparing to 30 healthy men. In validation-phage, relieved pelvic-pain symptom of 21 follow-up patients was found to be accompanied by significant down-regulation of miR-21-5p, miR-103a-3p and miR-141-3p. Particularly, ROC curve analysis indicated the highest area under ROC curve (AUC) was found for miR-21-5p (0.891), followed in order by miR-141-3p and miR-103a-3p. Our studies provided evidence that secretory miRNAs existed in EPS and dysregulated EPS-miRNAs were associated with prostatitis. In particular, miR-21-5p possessed a high classify-accuracy for IIIA CP/CPPS patients with significant pelvic pain.