Epidermal Microfluidic Electrochemical Detection System: Enhanced Sweat Sampling and Metabolite Detection
Epidermal Microfluidic Electrochemical Detection System: Enhanced Sweat Sampling and Metabolite Detection
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DOI：
10.1021/acssensors.7b00729
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发表时间：
2017-12-01
期刊：
影响因子：
8.9
通讯作者：
Wang, Joseph
中科院分区：
文献类型：
--
作者：
Martin, Aida;Kim, Jayoung;Wang, Joseph
尽管最近做出了巨大的努力，但无创汗液监测仍然远远没有实现其早期分析的承诺。在这里，我们描述了一种灵活的表皮微流体检测平台，通过光刻和丝网印刷技术的杂交制造，用于有效和快速的汗液采样和葡萄糖和乳酸水平的连续实时电化学监测。这种柔软的皮肤贴装设备明智地融合了芯片实验室和电化学检测技术，与小型化柔性电子板集成，用于向移动终端进行实时无线数据传输。装置设计和汗液流动条件的建模允许优化采样过程和微通道布局，以实现有吸引力的流体动力学和检测储液器的快速填充（从开始锻炼起8分钟内）。因此，可穿戴微型装置能够将有效的自然汗液泵送到包含酶修饰电极换能器的电化学检测室。所制造的装置可以容易地安装在表皮上而不妨碍佩戴者，并且显示出抵抗这种表皮磨损所预期的连续机械变形的弹性。使用相应的固定化氧化酶，从快速产生的汗液中的乳酸和葡萄糖的安培生物传感，在不同的健康受试者的自行车活动期间进行无线监测。这种监测汗液葡萄糖水平的能力为有效的糖尿病管理带来了新的可能性，而类似的乳酸监测为新的可穿戴健身应用铺平了道路。新的表皮微流体电化学检测策略代表了最近报道的比色汗液监测方法的一种有吸引力的替代方案，因此在实际健身或健康监测应用中具有相当大的前景。
Despite tremendous recent efforts, noninvasive sweat monitoring is still far from delivering its early analytical promise. Here, we describe a flexible epidermal microfluidic detection platform fabricated through hybridization of lithographic and screen-printed technologies, for efficient and fast sweat sampling and continuous, real-time electrochemical monitoring of glucose and lactate levels. This soft, skin-mounted device judiciously merges lab-on-a-chip and electrochemical detection technologies, integrated with a miniaturized flexible electronic board for real-time wireless data transmission to a mobile device. Modeling of the device design and sweat flow conditions allowed optimization of the sampling process and the microchannel layout for achieving attractive fluid dynamics and rapid filling of the detection reservoir (within 8 min from starting exercise). The wearable microdevice thus enabled efficient natural sweat pumping to the electrochemical detection chamber containing the enzyme-modified electrode transducers. The fabricated device can be easily mounted on the epidermis without hindrance to the wearer and displays resiliency against continuous mechanical deformation expected from such epidermal wear. Amperometric biosensing of lactate and glucose from the rapidly generated sweat, using the corresponding immobilized oxidase enzymes, was wirelessly monitored during cycling activity of different healthy subjects. This ability to monitor sweat glucose levels introduces new possibilities for effective diabetes management, while similar lactate monitoring paves the way for new wearable fitness applications. The new epidermal microfluidic electrochemical detection strategy represents an attractive alternative to recently reported colorimetric sweat-monitoring methods, and hence holds considerable promise for practical fitness or health monitoring applications.