Development of a recombinase polymerase amplification assay for rapid detection of Streptococcus suis type 2 in nasopharyngeal swab samples
Development of a recombinase polymerase amplification assay for rapid detection of Streptococcus suis type 2 in nasopharyngeal swab samples
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DOI：
10.1016/j.diagmicrobio.2021.115594
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发表时间：
2022-02-01
影响因子：
2.9
通讯作者：
Zhan, Dongbo
中科院分区：
文献类型：
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作者：
Jiang, Xiaowu;Zhu, Lexin;Zhan, Dongbo
猪链球菌血清2型（SS2）是一种新出现的人畜共患病原体，可在人和猪中引起严重感染和表现为败血症、脑膜炎甚至死亡的症状。本文旨在建立一种针对cps2J基因的实时重组酶聚合酶扩增（RT-RPA）检测SS2（或SS1/2）的新方法。评估RT-RPA结果的灵敏度和再现性，并与实时定量PCR （RT-qPCR）进行比较。建立的RT-RPA反应可在20分钟内完成，对3、4、5、7、9、14和31种主要猪链球菌感染血清型具有明显的特异性。RT-RPA的最低检测限为每次反应102个基因组DNA拷贝。在鼻咽拭子样品中检测RT-RPA的标本性能，灵敏度为97.5%，特异性为100%。因此，RT-RPA方法是一种快速、有潜力的SS2检测分子诊断工具。(C) 2021爱思唯尔公司版权所有。
Streptococcus suis serotype 2 (SS2), an emerging zoonotic pathogen, may induce severe infections and symptoms manifested as septicemia, meningitis and even death both in human and pigs. The aim of this article was to develop a new methodology as real-time recombinase polymerase amplification (RT-RPA) assay targeting cps2J gene for the detection of SS2 (or SS1/2). The sensitivity and reproducibility of RT-RPA results were evaluated and compared with a real-time quantitative PCR (RT-qPCR). The established RT-RPA reaction could be completed in 20 minutes with distinguishable specificity against the predominant S. suis infection serotypes of 3, 4, 5, 7, 9, 14, and 31. Lower detection limit for RT-RPA was 102 genomic DNA copies per reaction. The specimen performance of RT-RPA was tested in nasopharyngeal swab samples with the sensitivity and specificity as 97.5% and 100%, respectively. Thus, this RT-RPA method is a rapid and potential molecular diagnostic tool for SS2 detection. (C) 2021 Elsevier Inc. All rights reserved.