Artificial Analogues of Circulating Box C/D RNAs Induce Strong Innate Immune Response and MicroRNA Activation in Human Adenocarcinoma Cells
Artificial Analogues of Circulating Box C/D RNAs Induce Strong Innate Immune Response and MicroRNA Activation in Human Adenocarcinoma Cells
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DOI：
10.1007/978-3-319-42044-8_24
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Semenov, Dmitriy V.
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作者：
Stepanov, Grigory A.;Filippova, Julia A.;Semenov, Dmitriy V.
核仁小RNA（snoRNA）片段存在于人类血液中循环的各种非编码RNA（ncRNA）中。目前，这种无细胞sno衍生的RNA的功能还没有明确定义。这项工作的目的是确定由细胞外snoRNA控制的调节途径。为了确定人工snoRNA影响的分子靶标和途径，我们对用box C/D RNA转染的MCF-7人腺癌细胞进行了Illumina阵列分析。与对照细胞相比，发现与先天免疫应答和凋亡级联相关的基因在转染细胞中被激活。有趣的是，用人工box C/D snoRNA转染MCF-7细胞也增加了几种microRNA的转录，如mir-574，mir-599和mir-21。我们的数据表明，细胞外的snoRNA引入到人类细胞可能作为基因表达调节剂，与microRNA基因的激活是调控机制之一。
Fragments of small nucleolar RNAs (snoRNAs) were found among various non-coding RNAs (ncRNAs) circulating in human blood. Currently, the function of such cell-free sno-derived-RNAs is not clearly defined. This work is aimed at identifying regulatory pathways controlled by extracellular snoRNAs. In order to determine the molecular targets and pathways affected by artificial snoRNAs, we performed Illumina array analysis of MCF-7 human adenocarcinoma cells transfected with box C/D RNAs. The genes related to the innate immune response and apoptotic cascades were found to be activated in transfected cells compared with control cells. Intriguingly, the transfection of MCF-7 cells with artificial box C/D snoRNAs also increased the transcription of several microRNAs, such as mir-574, mir-599 and mir-21. Our data demonstrated that extracellular snoRNAs introduced into human cells may function as gene expression modulators, with activation of microRNA genes being one of the regulatory mechanisms.