Candidate Gene and MicroRNA Expression in Fetal Membranes and Preterm Delivery Risk
Candidate Gene and MicroRNA Expression in Fetal Membranes and Preterm Delivery Risk
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DOI：
10.1177/1933719115612925
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发表时间：
2016-06-01
影响因子：
2.9
通讯作者：
Williams, Michelle A.
中科院分区：
文献类型：
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作者：
Enquobahrie, Daniel A.;Hensley, Mark;Williams, Michelle A.
我们研究了候选基因和microRNA（miRNA）在羊膜和绒毛膜中的表达与早产（PTD）风险的关系。从胎盘中分离羊膜和绒毛膜，并在分娩时从足月分娩的参与者（N = 10）和在自然分娩（sPTL-PTD; N = 10）、胎膜早破（PPROM-PTD; N = 10）和先兆子痫（PE-PTD; N = 10）后早产的参与者中收集羊膜和绒毛膜。使用定量实时聚合酶链反应方法分析基因（金属蛋白酶[MMP] 2、MMP-9和MMP-1的组织抑制剂）和miRNA（miR-199 a *、-202*、-210、-214、-223和-338）的表达。使用调整后的多项逻辑回归模型计算相对风险比（RRR）、95%置信区间和P值。在对照组中，羊膜中miR-199 a *、miR-202 * 和miR-214的表达低于其在绒毛膜中的表达，而羊膜中miR-210的表达高于其在绒毛膜中的表达（所有P值均< .05）。在羊膜中，MMP-9表达与PTD风险相关（总体P值= 0.0092），MMP-9表达与PPROM-PTD风险正相关（RRR：31.10），与PE-PTD风险负相关（RRR：6.55e-6），尽管个体相关性无统计学意义。此外，在羊膜中，miR-210的表达（RRR：0.45;总体P值= .0039）与PE-PTD的风险呈负相关，miR-223与PTD的所有亚型呈负相关（总体P值= .0400）。羊膜和绒毛膜的miRNA表达不同。羊膜中MMP-9、miR-210和-223的表达与PTD风险相关。
We investigated candidate gene and microRNA (miRNA) expression in amnion and chorion in relation to risk of preterm delivery (PTD). Amnion and chorion were separated from placenta and collected at delivery from participants who delivered at term (N = 10) and from participants who delivered preterm following spontaneous labor (sPTL-PTD; N = 10), premature rupture of membranes (PPROM-PTD; N = 10), and preeclampsia (PE-PTD; N = 10). Expression of genes (metalloproteinase [MMP] 2, MMP-9, and tissue inhibitors of MMP-1) and miRNAs (miR-199a*, -202*, -210, -214, -223, and -338) was profiled using quantitative real-time polymerase chain reaction approaches. Adjusted multinomial logistic regression models were used to calculate relative risk ratios (RRR), 95% confidence intervals, and P values. Among controls, the expression of miR-199a*, -202*, and -214 was lower in the amnion compared with their expression in the chorion, whereas the expression of miR-210 was higher in the amnion compared with its expression in the chorion (all P values < .05). In the amnion, MMP-9 expression was associated with PTD risk (overall P value = .0092), and MMP-9 expression was positively associated with the risk of PPROM-PTD (RRR: 31.10) and inversely associated with the risk of PE-PTD (RRR:6.55e-6), although individual associations were not statistically significant. In addition, in the amnion, the expression of miR-210 (RRR: 0.45; overall P value = .0039) was inversely associated with the risk of PE-PTD, and miR-223 was inversely associated with all subtypes of PTD (overall P value = .0400). The amnion and chorion differ in their miRNA expression. The expression of MMP-9, miR-210, and -223 in the amnion is associated with PTD risk.